This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1fuu
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1fuu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fuu, resolution 2.5Å" /> '''YEAST INITIATION FACT...)
Next diff →
Revision as of 13:11, 20 November 2007
|
YEAST INITIATION FACTOR 4A
Overview
The eukaryotic translation initiation factor 4A (eIF4A) is a member of the, DEA(D/H)-box RNA helicase family, a diverse group of proteins that couples, an ATPase activity to RNA binding and unwinding. Previous work has, provided the structure of the amino-terminal, ATP-binding domain of eIF4A., Extending those results, we have solved the structure of the, carboxyl-terminal domain of eIF4A with data to 1.75 A resolution; it has a, parallel alpha-beta topology that superimposes, with minor variations, on, the structures and conserved motifs of the equivalent domain in other, distantly related helicases. Using data to 2.8 A resolution and molecular, replacement with the refined model of the carboxyl-terminal domain, we, have completed the structure of full-length eIF4A; it is a "dumbbell", structure consisting of two compact domains connected by an extended, linker. By using the structures of other helicases as a template, compact, structures can be modeled for eIF4A that suggest (i) helicase motif IV, binds RNA; (ii) Arg-298, which is conserved in the DEA(D/H)-box RNA, helicase family but is absent from many other helicases, also binds RNA;, and (iii) motifs V and VI "link" the carboxyl-terminal domain to the, amino-terminal domain through interactions with ATP and the DEA(D/H), motif, providing a mechanism for coupling ATP binding and hydrolysis with, conformational changes that modulate RNA binding.
About this Structure
1FUU is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase., Caruthers JM, Johnson ER, McKay DB, Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13080-5. PMID:11087862
Page seeded by OCA on Tue Nov 20 15:18:58 2007
