1g6v

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1g6v.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1g6v.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1g6v| PDB=1g6v | SCENE= }}
{{STRUCTURE_1g6v| PDB=1g6v | SCENE= }}
-
'''Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase'''
+
===Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase===
-
==Overview==
+
<!--
-
Detailed knowledge on antibody-antigen recognition is scarce given the unlimited antibody specificities of which only few have been investigated at an atomic level. We report the crystal structures of an antibody fragment derived from a camel heavy chain antibody against carbonic anhydrase, free and in complex with antigen. Surprisingly, this single-domain antibody interacts with nanomolar affinity with the antigen through its third hypervariable loop (19 amino acids long), providing a flat interacting surface of 620 A(2). For the first time, a single-domain antibody is observed with its first hypervariable loop adopting a type-1 canonical structure. The second hypervariable loop, of unique size due to a somatic mutation, reveals a regular beta-turn. The third hypervariable loop covers the remaining hypervariable loops and the side of the domain that normally interacts with the variable domain of the light chain. Specific amino acid substitutions and reoriented side chains reshape this side of the domain and increase its hydrophilicity. Of interest is the substitution of the conserved Trp-103 by Arg because it opens new perspectives to 'humanize' a camel variable domain of heavy chain of heavy chain antibody (VHH) or to 'camelize' a human or a mouse variable domain of heavy chain of conventional antibody (VH).
+
The line below this paragraph, {{ABSTRACT_PUBMED_11342547}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11342547 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11342547}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Complex]]
[[Category: Complex]]
[[Category: Immunoglobulin]]
[[Category: Immunoglobulin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:12:59 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:47:17 2008''

Revision as of 01:47, 1 July 2008

Image:1g6v.png

Template:STRUCTURE 1g6v

Complex of the camelid heavy-chain antibody fragment CAB-CA05 with bovine carbonic anhydrase

Template:ABSTRACT PUBMED 11342547

About this Structure

1G6V is a Single protein structure of sequence from Bos taurus and Camelus dromedarius. Full crystallographic information is available from OCA.

Reference

Antigen specificity and high affinity binding provided by one single loop of a camel single-domain antibody., Desmyter A, Decanniere K, Muyldermans S, Wyns L, J Biol Chem. 2001 Jul 13;276(28):26285-90. Epub 2001 May 7. PMID:11342547

Page seeded by OCA on Tue Jul 1 04:47:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1g6v"
Personal tools