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1g7o

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[[Image:1g7o.jpg|left|200px]]
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{{Seed}}
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[[Image:1g7o.png|left|200px]]
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{{STRUCTURE_1g7o| PDB=1g7o | SCENE= }}
{{STRUCTURE_1g7o| PDB=1g7o | SCENE= }}
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'''NMR SOLUTION STRUCTURE OF REDUCED E. COLI GLUTAREDOXIN 2'''
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===NMR SOLUTION STRUCTURE OF REDUCED E. COLI GLUTAREDOXIN 2===
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==Overview==
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Glutaredoxin 2 (Grx2) from Escherichia coli is distinguished from other glutaredoxins by its larger size, low overall sequence identity and lack of electron donor activity with ribonucleotide reductase. However, catalysis of glutathione (GSH)-dependent general disulfide reduction by Grx2 is extremely efficient. The high-resolution solution structure of E. coli Grx2 shows a two-domain protein, with residues 1 to 72 forming a classical "thioredoxin-fold" glutaredoxin domain, connected by an 11 residue linker to the highly helical C-terminal domain, residues 84 to 215. The active site, Cys9-Pro10-Tyr11-Cys12, is buried in the interface between the two domains, but Cys9 is solvent-accessible, consistent with its role in catalysis. The structures reveal the hither to unknown fact that Grx2 is structurally similar to glutathione-S-transferases (GST), although there is no obvious sequence homology. The similarity of these structures gives important insights into the functional significance of a new class of mammalian GST-like proteins, the single-cysteine omega class, which have glutaredoxin oxidoreductase activity rather than GSH-S-transferase conjugating activity. E. coli Grx 2 is structurally and functionally a member of this new expanding family of large glutaredoxins. The primary function of Grx2 as a GST-like glutaredoxin is to catalyze reversible glutathionylation of proteins with GSH in cellular redox regulation including stress responses.
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The line below this paragraph, {{ABSTRACT_PUBMED_11453697}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11453697 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11453697}}
==About this Structure==
==About this Structure==
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1G7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7O OCA].
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1G7O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G7O OCA].
==Reference==
==Reference==
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[[Category: Nmr]]
[[Category: Nmr]]
[[Category: Reduced form of glutaredoxin]]
[[Category: Reduced form of glutaredoxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:14:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:50:28 2008''

Revision as of 01:50, 1 July 2008

Image:1g7o.png

Template:STRUCTURE 1g7o

NMR SOLUTION STRUCTURE OF REDUCED E. COLI GLUTAREDOXIN 2

Template:ABSTRACT PUBMED 11453697

About this Structure

1G7O is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Solution structure of Escherichia coli glutaredoxin-2 shows similarity to mammalian glutathione-S-transferases., Xia B, Vlamis-Gardikas A, Holmgren A, Wright PE, Dyson HJ, J Mol Biol. 2001 Jul 20;310(4):907-18. PMID:11453697

Page seeded by OCA on Tue Jul 1 04:50:28 2008

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