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| | {{STRUCTURE_1g80| PDB=1g80 | SCENE= }} | | {{STRUCTURE_1g80| PDB=1g80 | SCENE= }} |
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| - | '''NMR SOLUTION STRUCTURE OF D(GCGTACGC)2'''
| + | ===NMR SOLUTION STRUCTURE OF D(GCGTACGC)2=== |
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| - | ==Overview==
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| - | The coupling between the conformational properties of double-stranded DNA and its internal dynamics has been examined. The solution structures of the isomeric DNA oligomers d(GCGTACGC)(2) (UM) and d(CGCTAGCG)(2) (CTSYM) were determined with (1)H NMR spectroscopy by utilizing distance restraints from total relaxation matrix analysis of NOESY cross-peak intensities in restrained molecular dynamics calculations. The root-mean-square deviation of the coordinates for the ensemble of structures was 0.13 A for UM and 0.49 A for CTSYM, with crystallographic equivalent R(c)=0.41 and 0.39 and sixth-root residual R(x)=0.11 and 0.10 for UM and CTSYM, respectively. Both UM and CTSYM are B-form with straight helical axes and show sequence-dependent variations in conformation. The internal dynamics of UM and CTSYM were previously determined by analysis of (13)C relaxation parameters in the context of the Lipari & Szabo model-free formalism. Helical parameters for the two DNA oligomers were examined for linear correlations with the order parameters (S(2)) of groups of (13)C spins in base-pairs and dinucleotide units of UM and CTSYM. Correlations were found for six interstrand base-pair parameters tip, y-displacement, inclination, buckle and stretch with various combinations of S(2) for atoms in Watson-Crick base-pairs and for two inter-base-pair parameters, rise and roll with various combinations of S(2) for atoms in dinucleotides. The correlations for the interstrand base-pair helical parameters indicate that the conformations of the deoxyribose residues of each strand are dynamically coupled. Also, the inter-base-pair separation has a profound effect on the local internal motions available to the DNA, supporting the idea that rise is a principal degree of freedom for DNA conformational variability. The correlations indicate collective atomic motions of spins that may represent specific motional modes in DNA, and that base sequence has a predictable effect on the relative order of groups of spins both in the bases and in the deoxyribose ring of the DNA backbone. These observations suggest that an important functional outcome of DNA base sequence is the modulation of both the conformation and dynamic behavior of the DNA backbone.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11254380}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11254380 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11254380}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G80 OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G80 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Phosphate conformation]] | | [[Category: Phosphate conformation]] |
| | [[Category: Psoralen]] | | [[Category: Psoralen]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:15:29 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:51:35 2008'' |
Revision as of 01:51, 1 July 2008
Template:STRUCTURE 1g80
NMR SOLUTION STRUCTURE OF D(GCGTACGC)2
Template:ABSTRACT PUBMED 11254380
About this Structure
Full experimental information is available from OCA.
Reference
Relationship of DNA structure to internal dynamics: correlation of helical parameters from NOE-based NMR solution structures of d(GCGTACGC)(2) and d(CGCTAGCG)(2) with (13)C order parameters implies conformational coupling in dinucleotide units., Isaacs RJ, Spielmann HP, J Mol Biol. 2001 Mar 23;307(2):525-40. PMID:11254380
Page seeded by OCA on Tue Jul 1 04:51:35 2008
