1g8x

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{{STRUCTURE_1g8x| PDB=1g8x | SCENE= }}
{{STRUCTURE_1g8x| PDB=1g8x | SCENE= }}
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'''STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR'''
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===STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR===
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==Overview==
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Molecular motors move unidirectionally along polymer tracks, producing movement and force in an ATP-dependent fashion. They achieve this by amplifying small conformational changes in the nucleotide-binding region into force-generating movements of larger protein domains. We present the 2.8 A resolution crystal structure of an artificial actin-based motor. By combining the catalytic domain of myosin II with a 130 A conformational amplifier consisting of repeats 1 and 2 of alpha-actinin, we demonstrate that it is possible to genetically engineer single-polypeptide molecular motors with precisely defined lever arm lengths and specific motile properties. Furthermore, our structure shows the consequences of mutating a conserved salt bridge in the nucleotide-binding region. Disruption of this salt bridge, which is known to severely inhibit ATP hydrolysis activity, appears to interfere with formation of myosin's catalytically active 'closed' conformation. Finally, we describe the structure of alpha-actinin repeats 1 and 2 as being composed of two rigid, triple-helical bundles linked by an uninterrupted alpha-helix. This fold is very similar to the previously described structures of alpha-actinin repeats 2 and 3, and alpha-spectrin repeats 16 and 17.
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The line below this paragraph, {{ABSTRACT_PUBMED_11226153}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11226153 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11226153}}
==About this Structure==
==About this Structure==
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[[Category: Myosin]]
[[Category: Myosin]]
[[Category: Protein engineering]]
[[Category: Protein engineering]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:17:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:54:10 2008''

Revision as of 01:54, 1 July 2008

Image:1g8x.png

Template:STRUCTURE 1g8x

STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR

Template:ABSTRACT PUBMED 11226153

About this Structure

1G8X is a Single protein structure of sequence from Dictyostelium discoideum. Full crystallographic information is available from OCA.

Reference

Structure of a genetically engineered molecular motor., Kliche W, Fujita-Becker S, Kollmar M, Manstein DJ, Kull FJ, EMBO J. 2001 Jan 15;20(1-2):40-6. PMID:11226153

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