1gjq

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{{STRUCTURE_1gjq| PDB=1gjq | SCENE= }}
{{STRUCTURE_1gjq| PDB=1gjq | SCENE= }}
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'''PSEUDOMONAS AERUGINOSA CD1 NITRITE REDUCTASE REDUCED CYANIDE COMPLEX'''
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===PSEUDOMONAS AERUGINOSA CD1 NITRITE REDUCTASE REDUCED CYANIDE COMPLEX===
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==Overview==
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Cyanide binding to fully reduced Pseudomonas aeruginosa cd(1) nitrite reductase (Pa cd(1) NiR) has been investigated for the wild-type enzyme and a site-directed mutant in which the active-site His369 was replaced by Ala. This mutation reduces the affinity toward cyanide (by approximately 13-fold) and especially decreases the rate of binding of cyanide to the reduced d(1) heme (by approximately 100-fold). The crystal structure of wild-type reduced Pa cd(1) NiR saturated with cyanide was determined to a resolution of 2.7 A. Cyanide binds to the iron of the d(1) heme, with an Fe-C-N angle of 168 degrees for both subunits of the dimer and only His369 is within hydrogen bonding distance of the nitrogen atom of the ligand. These results suggest that in Pa cd(1) NiR the invariant distal residue His369 plays a dominant role in controlling the binding of anionic ligands and allow the discussion of the mechanism of cyanide binding to the wild-type enzyme.
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(as it appears on PubMed at http://www.pubmed.gov), where 11829453 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11829453}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Reductase]]
[[Category: Reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:39:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:24:08 2008''

Revision as of 02:24, 1 July 2008

Image:1gjq.png

Template:STRUCTURE 1gjq

PSEUDOMONAS AERUGINOSA CD1 NITRITE REDUCTASE REDUCED CYANIDE COMPLEX

Template:ABSTRACT PUBMED 11829453

About this Structure

1GJQ is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Cyanide binding to cd(1) nitrite reductase from Pseudomonas aeruginosa: role of the active-site His369 in ligand stabilization., Sun W, Arese M, Brunori M, Nurizzo D, Brown K, Cambillau C, Tegoni M, Cutruzzola F, Biochem Biophys Res Commun. 2002 Feb 15;291(1):1-7. PMID:11829453

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