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| - | [[Image:1gmi.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1gmi| PDB=1gmi | SCENE= }} | | {{STRUCTURE_1gmi| PDB=1gmi | SCENE= }} |
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| - | '''STRUCTURE OF THE C2 DOMAIN FROM NOVEL PROTEIN KINASE C EPSILON'''
| + | ===STRUCTURE OF THE C2 DOMAIN FROM NOVEL PROTEIN KINASE C EPSILON=== |
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| - | ==Overview==
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| - | Protein kinase Cepsilon (PKCepsilon) is a member of the novel PKCs which are activated by acidic phospholipids, diacylglycerol and phorbol esters, but lack the calcium dependence of classical PKC isotypes. The crystal structures of the C2 domain of PKCepsilon, crystallized both in the absence and in the presence of the two acidic phospholipids, 1,2-dicaproyl-sn-phosphatidyl-l-serine (DCPS) and 1,2-dicaproyl-sn-phosphatidic acid (DCPA), have now been determined at 2.1, 1.7 and 2.8 A resolution, respectively. The central feature of the PKCepsilon-C2 domain structure is an eight-stranded, antiparallel, beta-sandwich with a type II topology similar to that of the C2 domains from phospholipase C and from novel PKCdelta. Despite the similar topology, important differences are found between the structures of C2 domains from PKCs delta and epsilon, suggesting they be considered as different PKC subclasses. Site-directed mutagenesis experiments and structural changes in the PKCepsilon-C2 domain from crystals with DCPS or DCPA indicate, though phospholipids were not visible in these structures, that loops joining strands beta1-beta2 and beta5-beta6 participate in the binding to anionic membranes. The different behavior in membrane-binding and activation between PKCepsilon and classical PKCs appears to originate in localized structural changes, which include a major reorganization of the region corresponding to the calcium binding pocket in classical PKCs. A mechanism is proposed for the interaction of the PKCepsilon-C2 domain with model membranes that retains basic features of the docking of C2 domains from classical, calcium-dependent, PKCs.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11518534}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11518534 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11518534}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Pkc epsilon.]] | | [[Category: Pkc epsilon.]] |
| | [[Category: X-ray]] | | [[Category: X-ray]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:45:07 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:31:01 2008'' |
Revision as of 02:31, 1 July 2008
Template:STRUCTURE 1gmi
STRUCTURE OF THE C2 DOMAIN FROM NOVEL PROTEIN KINASE C EPSILON
Template:ABSTRACT PUBMED 11518534
About this Structure
1GMI is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.
Reference
Structure of the C2 domain from novel protein kinase Cepsilon. A membrane binding model for Ca(2+)-independent C2 domains., Ochoa WF, Garcia-Garcia J, Fita I, Corbalan-Garcia S, Verdaguer N, Gomez-Fernandez JC, J Mol Biol. 2001 Aug 24;311(4):837-49. PMID:11518534
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