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| - | [[Image:1gnz.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1gnz.png|left|200px]] |
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| | {{STRUCTURE_1gnz| PDB=1gnz | SCENE= }} | | {{STRUCTURE_1gnz| PDB=1gnz | SCENE= }} |
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| - | '''LECTIN I-B4 FROM GRIFFONIA SIMPLICIFOLIA (GS I-B4)METAL FREE FORM'''
| + | ===LECTIN I-B4 FROM GRIFFONIA SIMPLICIFOLIA (GS I-B4)METAL FREE FORM=== |
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| - | ==Overview==
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| - | Seeds from the African legume shrub Griffonia simplicifolia contain several lectins. Among them the tetrameric lectin GS I-B(4) has strict specificity for terminal alpha Gal residues, whereas the closely related lectin GS I-A(4) can also bind to alpha GalNAc. These two lectins are commonly used as markers in histology or for research in xenotransplantation. To elucidate the basis for the fine difference in specificity, the amino acid sequences of both lectins have been determined and show 89% identity. The crystal structure of GS I-B(4), determined at 2.5-A resolution, reveals a new quaternary structure that has never been observed in other legume lectins. An unexpected loss of both Ca(2+) and Mn(2+) ions, which are necessary for carbohydrate binding in legume lectins, may be related to a particular amino acid sequence Pro-Glu-Pro in the metal binding loop. Comparison with demetallized concanavalin A reveals a different process for the loss of metal ions and for the subsequent loss of carbohydrate binding activity. The GS I-A x alpha GalNAc and GS I-B x alpha Gal complexes were constructed using homology modeling and docking approaches. The unusual presence of an aromatic amino acid at position 47 (Tyr in I-A and Trp in I-B) explains the strong preference for alpha-anomeric sugars in both isolectins. Alteration at one amino acid position, Ala(106) in I-A versus Glu(106) in I-B, is the basis for the observed specificities toward alpha GalNAc and alpha Gal.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11714720}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11714720 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11714720}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Glycoprotein]] | | [[Category: Glycoprotein]] |
| | [[Category: Lectin]] | | [[Category: Lectin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:48:20 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:34:59 2008'' |
Revision as of 02:35, 1 July 2008
Template:STRUCTURE 1gnz
LECTIN I-B4 FROM GRIFFONIA SIMPLICIFOLIA (GS I-B4)METAL FREE FORM
Template:ABSTRACT PUBMED 11714720
About this Structure
1GNZ is a Single protein structure of sequence from Griffonia simplicifolia. Full crystallographic information is available from OCA.
Reference
Isolectins I-A and I-B of Griffonia (Bandeiraea) simplicifolia. Crystal structure of metal-free GS I-B(4) and molecular basis for metal binding and monosaccharide specificity., Lescar J, Loris R, Mitchell E, Gautier C, Chazalet V, Cox V, Wyns L, Perez S, Breton C, Imberty A, J Biol Chem. 2002 Feb 22;277(8):6608-14. Epub 2001 Nov 19. PMID:11714720
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