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1go4

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{{STRUCTURE_1go4| PDB=1go4 | SCENE= }}
{{STRUCTURE_1go4| PDB=1go4 | SCENE= }}
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'''CRYSTAL STRUCTURE OF MAD1-MAD2 REVEALS A CONSERVED MAD2 BINDING MOTIF IN MAD1 AND CDC20.'''
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===CRYSTAL STRUCTURE OF MAD1-MAD2 REVEALS A CONSERVED MAD2 BINDING MOTIF IN MAD1 AND CDC20.===
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==Overview==
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The spindle checkpoint protein Mad1 recruits Mad2 to unattached kinetochores and is essential for Mad2-Cdc20 complex formation in vivo but not in vitro. The crystal structure of the Mad1-Mad2 complex reveals an asymmetric tetramer, with elongated Mad1 monomers parting from a coiled-coil to form two connected sub-complexes with Mad2. The Mad2 C-terminal tails are hinged mobile elements wrapping around the elongated ligands like molecular 'safety belts'. We show that Mad1 is a competitive inhibitor of the Mad2-Cdc20 complex, and propose that the Mad1-Mad2 complex acts as a regulated gate to control Mad2 release for Cdc20 binding. Mad1-Mad2 is strongly stabilized in the tetramer, but a 1:1 Mad1-Mad2 complex slowly releases Mad2 for Cdc20 binding, driven by favourable binding energies. Thus, the rate of Mad2 binding to Cdc20 during checkpoint activation may be regulated by conformational changes that destabilize the tetrameric Mad1-Mad2 assembly to promote Mad2 release. We also show that unlocking the Mad2 C-terminal tail is required for ligand release from Mad2, and that the 'safety belt' mechanism may prolong the lifetime of Mad2-ligand complexes.
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(as it appears on PubMed at http://www.pubmed.gov), where 12006501 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12006501}}
==About this Structure==
==About this Structure==
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[[Category: Mitotic spindle checkpoint]]
[[Category: Mitotic spindle checkpoint]]
[[Category: Nuclear pro]]
[[Category: Nuclear pro]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:48:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:35:25 2008''

Revision as of 02:35, 1 July 2008

Image:1go4.png

Template:STRUCTURE 1go4

CRYSTAL STRUCTURE OF MAD1-MAD2 REVEALS A CONSERVED MAD2 BINDING MOTIF IN MAD1 AND CDC20.

Template:ABSTRACT PUBMED 12006501

About this Structure

1GO4 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the tetrameric Mad1-Mad2 core complex: implications of a 'safety belt' binding mechanism for the spindle checkpoint., Sironi L, Mapelli M, Knapp S, De Antoni A, Jeang KT, Musacchio A, EMBO J. 2002 May 15;21(10):2496-506. PMID:12006501

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