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1goi

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[[Image:1goi.gif|left|200px]]
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{{STRUCTURE_1goi| PDB=1goi | SCENE= }}
{{STRUCTURE_1goi| PDB=1goi | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE D140N MUTANT OF CHITINASE B FROM SERRATIA MARCESCENS AT 1.45 A RESOLUTION'''
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===CRYSTAL STRUCTURE OF THE D140N MUTANT OF CHITINASE B FROM SERRATIA MARCESCENS AT 1.45 A RESOLUTION===
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==Overview==
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The crystal structure of the inactive D140N mutant of Serratia marcescens was refined to 1.45 A resolution. The structure of the mutant was essentially identical to that of the wild type, with the exception of a rotation of Asp142 in the catalytic centre. In the mutant, this residue interacts with the catalytic acid (Glu144) and not with residue 140 as in the wild type. Thus, the 500-fold decrease in activity in the D140N mutant seems to be largely mediated by an effect on Asp142, confirming the crucial role of the latter residue in catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 11807282 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11807282}}
==About this Structure==
==About this Structure==
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[[Category: Glycosidase]]
[[Category: Glycosidase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:49:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:36:31 2008''

Revision as of 02:36, 1 July 2008

Image:1goi.png

Template:STRUCTURE 1goi

CRYSTAL STRUCTURE OF THE D140N MUTANT OF CHITINASE B FROM SERRATIA MARCESCENS AT 1.45 A RESOLUTION

Template:ABSTRACT PUBMED 11807282

About this Structure

1GOI is a Single protein structure of sequence from Serratia marcescens. Full crystallographic information is available from OCA.

Reference

Structure of the D140N mutant of chitinase B from Serratia marcescens at 1.45 A resolution., Kolstad G, Synstad B, Eijsink VG, van Aalten DM, Acta Crystallogr D Biol Crystallogr. 2002 Feb;58(Pt 2):377-9. Epub 2002, Jan 24. PMID:11807282

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