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| | {{STRUCTURE_1gpc| PDB=1gpc | SCENE= }} | | {{STRUCTURE_1gpc| PDB=1gpc | SCENE= }} |
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| - | '''CORE GP32, DNA-BINDING PROTEIN'''
| + | ===CORE GP32, DNA-BINDING PROTEIN=== |
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| - | ==Overview==
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| - | The single-stranded DNA (ssDNA) binding protein gp32 from bacteriophage T4 is essential for T4 DNA replication, recombination and repair. In vivo gp32 binds ssDNA as the replication fork advances and stimulates replisome processivity and accuracy by a factor of several hundred. Gp32 binding affects nearly every major aspect of DNA metabolism. Among its important functions are: (1) configuring ssDNA templates for efficient use by the replisome including DNA polymerase; (2) melting out adventitious secondary structures; (3) protecting exposed ssDNA from nucleases; and (4) facilitating homologous recombination by binding ssDNA during strand displacement. We have determined the crystal structure of the gp32 DNA binding domain complexed to ssDNA at 2.2 A resolution. The ssDNA binding cleft comprises regions from three structural subdomains and includes a positively charged surface that runs parallel to a series of hydrophobic pockets formed by clusters of aromatic side chains. Although only weak electron density is seen for the ssDNA, it indicates that the phosphate backbone contacts an electropositive cleft of the protein, placing the bases in contact with the hydrophobic pockets. The DNA mobility implied by the weak electron density may reflect the role of gp32 as a sequence-independent ssDNA chaperone allowing the largely unstructured ssDNA to slide freely through the cleft. | + | The line below this paragraph, {{ABSTRACT_PUBMED_7630406}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7630406 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_7630406}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Ssb]] | | [[Category: Ssb]] |
| | [[Category: Zinc binding domain]] | | [[Category: Zinc binding domain]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:51:14 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:38:35 2008'' |
Revision as of 02:38, 1 July 2008
Template:STRUCTURE 1gpc
CORE GP32, DNA-BINDING PROTEIN
Template:ABSTRACT PUBMED 7630406
About this Structure
1GPC is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Crystal structure of a replication fork single-stranded DNA binding protein (T4 gp32) complexed to DNA., Shamoo Y, Friedman AM, Parsons MR, Konigsberg WH, Steitz TA, Nature. 1995 Jul 27;376(6538):362-6. PMID:7630406
Page seeded by OCA on Tue Jul 1 05:38:35 2008
