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| - | [[Image:1gq2.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1gq2| PDB=1gq2 | SCENE= }} | | {{STRUCTURE_1gq2| PDB=1gq2 | SCENE= }} |
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| - | '''MALIC ENZYME FROM PIGEON LIVER'''
| + | ===MALIC ENZYME FROM PIGEON LIVER=== |
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| - | ==Overview==
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| - | Malic enzymes are widely distributed in nature, and have important biological functions. They catalyze the oxidative decarboxylation of malate to produce pyruvate and CO(2) in the presence of divalent cations (Mg(2+), Mn(2+)). Most malic enzymes have a clear selectivity for the dinucleotide cofactor, being able to use either NAD(+) or NADP(+), but not both. Structural studies of the human mitochondrial NAD(+)-dependent malic enzyme established that malic enzymes belong to a new class of oxidative decarboxylases. Here we report the crystal structure of the pigeon cytosolic NADP(+)-dependent malic enzyme, in a closed form, in a quaternary complex with NADP(+), Mn(2+), and oxalate. This represents the first structural information on an NADP(+)-dependent malic enzyme. Despite the sequence conservation, there are large differences in several regions of the pigeon enzyme structure compared to the human enzyme. One region of such differences is at the binding site for the 2'-phosphate group of the NADP(+) cofactor, which helps define the cofactor selectivity of the enzymes. Specifically, the structural information suggests Lys362 may have an important role in the NADP(+) selectivity of the pigeon enzyme, confirming our earlier kinetic observations on the K362A mutant. Our structural studies also revealed differences in the organization of the tetramer between the pigeon and the human enzymes, although the pigeon enzyme still obeys 222 symmetry.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11790843}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11790843 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11790843}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Nadp-dependent]] | | [[Category: Nadp-dependent]] |
| | [[Category: Pigeon liver]] | | [[Category: Pigeon liver]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:52:37 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 05:51:53 2008'' |
Revision as of 02:52, 1 July 2008
Template:STRUCTURE 1gq2
MALIC ENZYME FROM PIGEON LIVER
Template:ABSTRACT PUBMED 11790843
About this Structure
1GQ2 is a Single protein structure of sequence from Columba livia. Full crystallographic information is available from OCA.
Reference
Structural studies of the pigeon cytosolic NADP(+)-dependent malic enzyme., Yang Z, Zhang H, Hung HC, Kuo CC, Tsai LC, Yuan HS, Chou WY, Chang GG, Tong L, Protein Sci. 2002 Feb;11(2):332-41. PMID:11790843
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