6ebx
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(New page: 200px<br /><applet load="6ebx" size="450" color="white" frame="true" align="right" spinBox="true" caption="6ebx, resolution 1.7Å" /> '''STRUCTURE DETERMINATI...)
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Revision as of 13:24, 20 November 2007
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STRUCTURE DETERMINATION OF A DIMERIC FORM OF ERABUTOXIN B, CRYSTALLIZED FROM THIOCYANATE SOLUTION
Overview
Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four, disulfide bridges, was crystallized in a new orthorhombic form by using, thiocyanate as crystallizing agent. The space group is P2(1)2(1)2(1) with, a = 53.36 (4), b = 40.89 (4), c = 55.71 (5) A, V = 121533.1 A and Z = 8., X-ray diffraction data were recorded at the LURE synchrotron facility, (lambda = 1.405 A). The structure was solved by molecular replacement and, shows a dimeric association through an anti-parallel beta-sheet around the, twofold non-crystallographic axis. The two independent molecules, one SCN-, ion and 97 associated water molecules were refined by molecular dynamics, and annealing techniques to R = 19.6% (10,913 Fobs, resolution 5-1.7 A)., The thiocyanate ion is located at the interface of the dimer and close to, the non-crystallographic twofold axis.
About this Structure
6EBX is a Single protein structure of sequence from Laticauda semifasciata with SCN as ligand. Full crystallographic information is available from OCA.
Reference
Structure determination of a dimeric form of erabutoxin-b, crystallized from a thiocyanate solution., Saludjian P, Prange T, Navaza J, Menez R, Guilloteau JP, Ries-Kautt M, Ducruix A, Acta Crystallogr B. 1992 Aug 1;48 ( Pt 4):520-31. PMID:1418823
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