1gza

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1gza.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1gza.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1gza| PDB=1gza | SCENE= }}
{{STRUCTURE_1gza| PDB=1gza | SCENE= }}
-
'''PEROXIDASE'''
+
===PEROXIDASE===
-
==Overview==
+
<!--
-
The site and characteristics of iodide binding to Arthromyces ramosus peroxidase were examined by x-ray crystallographic analysis, 1H and 127I NMR, and kinetic studies. X-ray analysis of an A. ramosus peroxidase crystal soaked in a KI solution at pH 5.5 showed that a single iodide ion is located at the entrance of the access channel to the distal side of the heme and lies between the two peptide segments, Phe90-Pro91-Ala92 and Ser151-Leu152-Ile153, 12.8 A from the heme iron. The distances between the iodide ion and heme peripheral methyl groups were all more than 10 A. The findings agree with the results obtained with 1H NMR in which the chemical shift and intensity of the methyl groups in the hyperfine shift region of A. ramosus peroxidase were hardly affected by the addition of iodide, unlike the case of horseradish peroxidase. Moreover, 127I NMR and steady-state kinetics showed that the binding of iodide depends on protonation of an amino acid residue with a pKa of about 5.3, which presumably is the distal histidine (His56), 7.8 A away from the iodide ion. The mechanism of electron transfer from the iodide ion to the heme iron is discussed on the basis of these findings.
+
The line below this paragraph, {{ABSTRACT_PUBMED_9038188}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 9038188 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_9038188}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Peroxidase]]
[[Category: Peroxidase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:12:33 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:21:33 2008''

Revision as of 03:21, 1 July 2008

Image:1gza.png

Template:STRUCTURE 1gza

PEROXIDASE

Template:ABSTRACT PUBMED 9038188

About this Structure

1GZA is a Single protein structure of sequence from Eukaryota. Full crystallographic information is available from OCA.

Reference

Binding of iodide to Arthromyces ramosus peroxidase investigated with X-ray crystallographic analysis, 1H and 127I NMR spectroscopy, and steady-state kinetics., Fukuyama K, Sato K, Itakura H, Takahashi S, Hosoya T, J Biol Chem. 1997 Feb 28;272(9):5752-6. PMID:9038188

Page seeded by OCA on Tue Jul 1 06:21:33 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gza"
Personal tools