1fzp
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(New page: 200px<br /><applet load="1fzp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fzp, resolution 2.95Å" /> '''CRYSTAL STRUCTURES O...)
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Revision as of 13:26, 20 November 2007
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CRYSTAL STRUCTURES OF SARA: A PLEIOTROPIC REGULATOR OF VIRULENCE GENES IN S. AUREUS
Overview
Staphylococcus aureus is a major human pathogen, the potency of which can, be attributed to the regulated expression of an impressive array of, virulence determinants. A key pleiotropic transcriptional regulator of, these virulence factors is SarA, which is encoded by the sar, (staphylococcal accessory regulator) locus. SarA was characterized, initially as an activator of a second virulence regulatory locus, agr, through its interaction with a series of heptad repeats (AGTTAAG) within, the agr promoter. Subsequent DNA-binding studies have revealed that SarA, binds readily to multiple AT-rich sequences of variable lengths. Here we, describe the crystal structure of SarA and a SarA-DNA complex at, resolutions of 2.50 A and 2.95 A, respectively. SarA has a fold consisting, of a four-helix core region and 'inducible regions' comprising a, beta-hairpin and a carboxy-terminal loop. On binding DNA, the inducible, regions undergo marked conformational changes, becoming part of extended, and distorted alpha-helices, which encase the DNA. SarA recognizes an, AT-rich site in which the DNA is highly overwound and adopts a D-DNA-like, conformation by indirect readout. These structures thus provide insight, into SarA-mediated transcription regulation.
About this Structure
1FZP is a Single protein structure of sequence from Staphylococcus aureus with CA as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structures of SarA, a pleiotropic regulator of virulence genes in S. aureus., Schumacher MA, Hurlburt BK, Brennan RG, Nature. 2001 Jan 11;409(6817):215-9. PMID:11196648
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