2ius

From Proteopedia

---

Revision as of 13:26, 20 November 2007

proteopedia linkproteopedia link

spinqualitylabelsall models 2ius, resolution 2.7Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

E. COLI FTSK MOTOR DOMAIN

Overview

FtsK is a DNA translocase that coordinates chromosome segregation and cell, division in bacteria. In addition to its role as activator of XerCD, site-specific recombination, FtsK can translocate double-stranded DNA, (dsDNA) rapidly and directionally and reverse direction. We present, crystal structures of the FtsK motor domain monomer, showing that it has a, RecA-like core, the FtsK hexamer, and also showing that it is a ring with, a large central annulus and a dodecamer consisting of two hexamers, head, to head. Electron microscopy (EM) demonstrates the DNA-dependent existence, of hexamers in solution and shows that duplex DNA passes through the, middle of each ring. Comparison of FtsK monomer structures from two, different crystal forms highlights a conformational change that we propose, is the structural basis for a rotary inchworm mechanism of DNA, translocation.

About this Structure

2IUS is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Double-stranded DNA translocation: structure and mechanism of hexameric FtsK., Massey TH, Mercogliano CP, Yates J, Sherratt DJ, Lowe J, Mol Cell. 2006 Aug;23(4):457-69. PMID:16916635

Page seeded by OCA on Tue Nov 20 15:33:17 2007