1h34

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{{STRUCTURE_1h34| PDB=1h34 | SCENE= }}
{{STRUCTURE_1h34| PDB=1h34 | SCENE= }}
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'''CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR'''
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===CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR===
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==Overview==
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SAD (single-wavelength anomalous diffraction) has enormous potential for phasing proteins using only the anomalous signal of the almost ubiquitous native sulfur, but requires extremely precise data. The previously unknown structure of the lima bean trypsin inhibitor (LBTI) was solved using highly redundant data collected to 3 A using a CCD detector with a rotating-anode generator and three-circle goniometer. The seven 'super-S' atoms (disulfide bridges) were located by dual-space recycling with SHELXD and the high solvent content enabled the density-modification program SHELXE to generate high-quality maps despite the modest resolution. Subsequently, a 2.05 A synchrotron data set was collected and used for further phase extension and structure refinement.
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The line below this paragraph, {{ABSTRACT_PUBMED_12554963}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 12554963 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12554963}}
==About this Structure==
==About this Structure==
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[[Category: Bowman-birk-type proteinase inhibitor]]
[[Category: Bowman-birk-type proteinase inhibitor]]
[[Category: Serine protease inhibitor]]
[[Category: Serine protease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:22:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:32:46 2008''

Revision as of 03:32, 1 July 2008

Image:1h34.png

Template:STRUCTURE 1h34

CRYSTAL STRUCTURE OF LIMA BEAN TRYPSIN INHIBITOR

Template:ABSTRACT PUBMED 12554963

About this Structure

1H34 is a Single protein structure of sequence from Phaseolus lunatus. Full crystallographic information is available from OCA.

Reference

In-house phase determination of the lima bean trypsin inhibitor: a low-resolution sulfur-SAD case., Debreczeni JE, Bunkoczi G, Girmann B, Sheldrick GM, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):393-5. Epub 2003, Jan 23. PMID:12554963

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