1h3j

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{{STRUCTURE_1h3j| PDB=1h3j | SCENE= }}
{{STRUCTURE_1h3j| PDB=1h3j | SCENE= }}
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'''STRUCTURE OF RECOMBINANT COPRINUS CINEREUS PEROXIDASE DETERMINED TO 2.0 A'''
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===STRUCTURE OF RECOMBINANT COPRINUS CINEREUS PEROXIDASE DETERMINED TO 2.0 A===
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==Overview==
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The structure of the peroxidase from Coprinus cinereus (CiP) has been determined in three different space groups and crystalline environments. Two of these are of the recombinant glycosylated form (rCiP), which crystallized in space groups P2(1)2(1)2(1) and C2. The third crystal form was obtained from a variant of CiP in which the glycosylation sites have been removed (rCiPON). It crystallizes in space group P2(1) with beta approximately 90 degrees; the structure was determined from room-temperature data and low-temperature data obtained from twinned crystals. Two independent molecules of CiP related by non-crystallographic symmetry are contained in the three crystal forms. The packing in the two structures of the glycosylated form of rCiP is closely related, but differs from the packing in the unglycosylated rCiPON. A database search based on small-molecule porphinato iron (III) complexes has been performed and related to observations of the spin states and coordination numbers of the iron ion. The room-temperature structures of CiP and one structure of the almost identical peroxidase from Arthromyces ramosus (ARP) have been used to identify 66 conserved water molecules and to assign a structural role to most of them.
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(as it appears on PubMed at http://www.pubmed.gov), where 12777760 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12777760}}
==About this Structure==
==About this Structure==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Peroxidase]]
[[Category: Peroxidase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:33:58 2008''

Revision as of 03:33, 1 July 2008

Image:1h3j.png

Template:STRUCTURE 1h3j

STRUCTURE OF RECOMBINANT COPRINUS CINEREUS PEROXIDASE DETERMINED TO 2.0 A

Template:ABSTRACT PUBMED 12777760

About this Structure

1H3J is a Single protein structure of sequence from Coprinopsis cinerea. Full crystallographic information is available from OCA.

Reference

Impact of the physical and chemical environment on the molecular structure of Coprinus cinereus peroxidase., Houborg K, Harris P, Petersen J, Rowland P, Poulsen JC, Schneider P, Vind J, Larsen S, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):989-96. Epub 2003, May 23. PMID:12777760

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