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1h3l

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{{STRUCTURE_1h3l| PDB=1h3l | SCENE= }}
{{STRUCTURE_1h3l| PDB=1h3l | SCENE= }}
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'''N-TERMINAL FRAGMENT OF SIGR FROM STREPTOMYCES COELICOLOR'''
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===N-TERMINAL FRAGMENT OF SIGR FROM STREPTOMYCES COELICOLOR===
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==Overview==
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The extracytoplasmic function (ECF) sigma factor sigma(R) is a global regulator of redox homeostasis in the antibiotic-producing bacterium Streptomyces coelicolor, with a similar role in other actinomycetes such as Mycobacterium tuberculosis. Normally maintained in an inactive state by its bound anti-sigma factor RsrA, sigma(R) dissociates in response to intracellular disulphide-stress to direct core RNA polymerase to transcribe genes, such as trxBA and trxC that encode the enzymes of the thioredoxin disulphide reductase pathway, that re-establish redox homeostasis. Little is known about where RsrA binds on sigma(R) or how it suppresses sigma(R)-dependent transcriptional activity. Using a combination of proteolysis, surface-enhanced laser desorption ionisation mass spectrometry and pull-down assays we identify an N-terminal, approximately 10kDa domain (sigma(RN)) that encompasses region 2 of sigma(R) that represents the major RsrA binding site. We show that sigma(RN) inhibits transcription by an unrelated sigma factor and that this inhibition is relieved by RsrA binding, reaffirming that region 2 is involved in binding to core RNA polymerase but also demonstrating that the likely mechanism by which RsrA inhibits sigma(R) activity is by blocking this association. We also report the 2.4A resolution crystal structure of sigma(RN) that reveals extensive structural conservation with the equivalent region of sigma(70) from Escherichia coli as well as with the cyclin-box, a domain-fold found in the eukaryotic proteins TFIIB and cyclin A. sigma(RN) has a propensity to aggregate, due to steric complementarity of oppositely charged surfaces on the domain, but this is inhibited by RsrA, an observation that suggests a possible mode of action for RsrA which we compare to other well-studied sigma factor-anti-sigma factor systems.
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{{ABSTRACT_PUBMED_12381317}}
==About this Structure==
==About this Structure==
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[[Category: Dna-directed rna polymerase]]
[[Category: Dna-directed rna polymerase]]
[[Category: Sigma factor]]
[[Category: Sigma factor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:34:02 2008''

Revision as of 03:34, 1 July 2008

Image:1h3l.png

Template:STRUCTURE 1h3l

N-TERMINAL FRAGMENT OF SIGR FROM STREPTOMYCES COELICOLOR

Template:ABSTRACT PUBMED 12381317

About this Structure

1H3L is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Identification and structure of the anti-sigma factor-binding domain of the disulphide-stress regulated sigma factor sigma(R) from Streptomyces coelicolor., Li W, Stevenson CE, Burton N, Jakimowicz P, Paget MS, Buttner MJ, Lawson DM, Kleanthous C, J Mol Biol. 2002 Oct 18;323(2):225-36. PMID:12381317

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