1h8h

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{{Seed}}
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{{STRUCTURE_1h8h| PDB=1h8h | SCENE= }}
{{STRUCTURE_1h8h| PDB=1h8h | SCENE= }}
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'''BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP'''
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===BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP===
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==Overview==
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Analysis of tryptophan mutants of F(1)-ATPase from Escherichia coli [Lobau et al. (1997) FEBS Lett. 404, 15-18] suggested that nucleotide concentrations used to grow crystals for the determination of the structure of bovine F(1)-ATPase [Abrahams et al. (1994) Nature 370, 621-628] would be sufficient to occupy only two catalytic sites, and that higher concentrations of nucleotide would result in all three sites being occupied. We have determined the structure of bovine F(1)-ATPase at 2.9 A resolution with crystals grown in the presence of 5 mM AMPPNP and 5 microM ADP. Similar to previous structures of bovine F(1)-ATPase determined with crystals grown in the presence of lower nucleotide concentrations, only two beta-subunits have bound nucleotide and the third subunit remains empty.
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(as it appears on PubMed at http://www.pubmed.gov), where 11297725 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11297725}}
==About this Structure==
==About this Structure==
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[[Category: F1-atpase]]
[[Category: F1-atpase]]
[[Category: F1fo atp synthase]]
[[Category: F1fo atp synthase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:34:14 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 06:48:20 2008''

Revision as of 03:48, 1 July 2008

Image:1h8h.png

Template:STRUCTURE 1h8h

BOVINE MITOCHONDRIAL F1-ATPASE CRYSTALLISED IN THE PRESENCE OF 5MM AMPPNP

Template:ABSTRACT PUBMED 11297725

About this Structure

1H8H is a Protein complex structure of sequences from Bos taurus. Full crystallographic information is available from OCA.

Reference

The structure and nucleotide occupancy of bovine mitochondrial F(1)-ATPase are not influenced by crystallisation at high concentrations of nucleotide., Menz RI, Leslie AG, Walker JE, FEBS Lett. 2001 Apr 6;494(1-2):11-4. PMID:11297725

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