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| | {{STRUCTURE_1ha8| PDB=1ha8 | SCENE= }} | | {{STRUCTURE_1ha8| PDB=1ha8 | SCENE= }} |
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| - | '''PHEROMONE ER-23 FROM EUPLOTES RAIKOVI'''
| + | ===PHEROMONE ER-23 FROM EUPLOTES RAIKOVI=== |
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| - | ==Overview==
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| - | The NMR solution structure of the 51 residue pheromone Er-23 from the ciliated protozoan Euplotes raikovi (Er) was calculated with the torsion angle dynamics program DYANA from 582 nuclear Overhauser enhancement (NOE) upper limit distance constraints, 46 dihedral angle constraints and 30 disulfide bond constraints. The disulfide bridges had not been assigned by chemical methods, and initially were assigned tentatively on the basis of inspection of the positioning of the Cys sulfhydryl groups in a bundle of 20 conformers that was calculated without disulfide bond constraints. The assignment of disulfide bridges was then validated by structure calculations that assessed the compatibility of plausible alternative Cys-Cys disulfide combinations with the input of NOE upper distance constraints and dihedral angle constraints. For a group of 20 conformers used to characterize the solution structure, the average pairwise root-mean-square distances from the mean coordinates calculated for the backbone heavy atoms N, C(alpha) and C' of resideus 1-51 is 0.38 A. The molecular architecture consists of a three-dimensional arrangement of five helices comprised of residues 2-8, 14-17, 26-29, 34-36 and 38-47, with five disulfide bridges in the positions 3-24, 6-16, 13-47, 27-40, and 35-51, which has so far not been represented in the Protein Data Bank. Er-23 is unique among presently known Er-pheromones with respect to size, sequence, the number of disulfide bonds and the three-dimensional structure, thus providing a new structural basis for rationalizing the physiological functions of this protein family. | + | The line below this paragraph, {{ABSTRACT_PUBMED_11700049}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11700049 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11700049}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1HA8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HA8 OCA]. | + | 1HA8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Euplotes_raikovi Euplotes raikovi]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HA8 OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Zahn, R.]] | | [[Category: Zahn, R.]] |
| | [[Category: Pheromone]] | | [[Category: Pheromone]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:37:51 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 07:53:24 2008'' |
Revision as of 04:53, 1 July 2008
Template:STRUCTURE 1ha8
PHEROMONE ER-23 FROM EUPLOTES RAIKOVI
Template:ABSTRACT PUBMED 11700049
About this Structure
1HA8 is a Single protein structure of sequence from Euplotes raikovi. Full experimental information is available from OCA.
Reference
NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds., Zahn R, Damberger F, Ortenzi C, Luporini P, Wuthrich K, J Mol Biol. 2001 Nov 9;313(5):923-31. PMID:11700049
Page seeded by OCA on Tue Jul 1 07:53:24 2008
