1haz

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{{STRUCTURE_1haz| PDB=1haz | SCENE= }}
{{STRUCTURE_1haz| PDB=1haz | SCENE= }}
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'''SNAPSHOTS OF SERINE PROTEASE CATALYSIS: (C) ACYL-ENZYME INTERMEDIATE BETWEEN PORCINE PANCREATIC ELASTASE AND HUMAN BETA-CASOMORPHIN-7 JUMPED TO PH 9 FOR 1 MINUTE'''
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===SNAPSHOTS OF SERINE PROTEASE CATALYSIS: (C) ACYL-ENZYME INTERMEDIATE BETWEEN PORCINE PANCREATIC ELASTASE AND HUMAN BETA-CASOMORPHIN-7 JUMPED TO PH 9 FOR 1 MINUTE===
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==Overview==
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Studies on the catalytic mechanism and inhibition of serine proteases are widely used as paradigms for teaching enzyme catalysis. Ground-breaking work on the structures of chymotrypsin and subtilisin led to the idea of a conserved catalytic triad formed by the active site Ser, His and Asp residues. An oxyanion hole, consisting of the peptide amide of the active site serine and a neighbouring glycine, was identified, and hydrogen bonding in the oxyanion hole was suggested to stabilize the two proposed tetrahedral intermediates on the catalytic pathway. Here we show electron density changes consistent with the formation of a tetrahedral intermediate during the hydrolysis of an acyl-enzyme complex formed between a natural heptapeptide and elastase. No electron density for an enzyme-product complex was observed. The structures also suggest a mechanism for the synchronization of hydrolysis and peptide release triggered by the conversion of the sp2 hybridized carbonyl carbon to an sp3 carbon in the tetrahedral intermediate. This affects the location of the peptide in the active site cleft, triggering the collapse of a hydrogen bonding network between the peptide and the beta-sheet of the active site.
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The line below this paragraph, {{ABSTRACT_PUBMED_11473259}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11473259 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11473259}}
==About this Structure==
==About this Structure==
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[[Category: Wright, P A.]]
[[Category: Wright, P A.]]
[[Category: Serine proteinase]]
[[Category: Serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 07:55:16 2008''

Revision as of 04:55, 1 July 2008

Image:1haz.png

Template:STRUCTURE 1haz

SNAPSHOTS OF SERINE PROTEASE CATALYSIS: (C) ACYL-ENZYME INTERMEDIATE BETWEEN PORCINE PANCREATIC ELASTASE AND HUMAN BETA-CASOMORPHIN-7 JUMPED TO PH 9 FOR 1 MINUTE

Template:ABSTRACT PUBMED 11473259

About this Structure

1HAZ is a Protein complex structure of sequences from Homo sapiens and Sus scrofa. Full crystallographic information is available from OCA.

Reference

X-ray snapshots of serine protease catalysis reveal a tetrahedral intermediate., Wilmouth RC, Edman K, Neutze R, Wright PA, Clifton IJ, Schneider TR, Schofield CJ, Hajdu J, Nat Struct Biol. 2001 Aug;8(8):689-94. PMID:11473259

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