1hcj

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{{STRUCTURE_1hcj| PDB=1hcj | SCENE= }}
{{STRUCTURE_1hcj| PDB=1hcj | SCENE= }}
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'''PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN'''
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===PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN===
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==Overview==
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Wild type green fluorescent protein (GFP) from Aequorea victoria absorbs predominantly at 398 nm. Illumination with UV (254 nm) or visible (390 nm) light transforms this state (GFP(398)) into one absorbing at 483 nm (GFP(483)). Here we show that this photoconversion of GFP is a one-photon process that is paralleled by decarboxylation of Glu 222. We propose a mechanism in which decarboxylation is due to electron transfer between the gamma-carboxylate of Glu 222 and the p-hydroxybenzylidene-imidazolidinone chromophore of GFP, followed by reverse transfer of an electron and a proton to the remaining carbon side chain atom of Glu 222. Oxidative decarboxylation of a gamma-carboxylate represents a new type of posttranslational modification that may also occur in enzymes with high-potential reaction intermediates.
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(as it appears on PubMed at http://www.pubmed.gov), where 11740505 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11740505}}
==About this Structure==
==About this Structure==
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[[Category: Fluorescent protein]]
[[Category: Fluorescent protein]]
[[Category: Luminescence]]
[[Category: Luminescence]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:42:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 07:59:20 2008''

Revision as of 04:59, 1 July 2008

Image:1hcj.png

Template:STRUCTURE 1hcj

PHOTOPRODUCT OF THE WILD-TYPE AEQUOREA VICTORIA GREEN FLUORESCENT PROTEIN

Template:ABSTRACT PUBMED 11740505

About this Structure

1HCJ is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Phototransformation of green fluorescent protein with UV and visible light leads to decarboxylation of glutamate 222., van Thor JJ, Gensch T, Hellingwerf KJ, Johnson LN, Nat Struct Biol. 2002 Jan;9(1):37-41. PMID:11740505

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