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1hg9

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[[Image:1hg9.gif|left|200px]]
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{{STRUCTURE_1hg9| PDB=1hg9 | SCENE= }}
{{STRUCTURE_1hg9| PDB=1hg9 | SCENE= }}
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'''SOLUTION STRUCTURE OF DNA:RNA HYBRID'''
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===SOLUTION STRUCTURE OF DNA:RNA HYBRID===
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==Overview==
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We have used NMR and CD spectroscopy to study the conformations of modified oligonucleotides (locked nucleic acid, LNA) containing a conformationally restricted nucleotide (T(L)) with a 2'-O,4'-C-methylene bridge. We have investigated two LNA:RNA duplexes, d(CTGAT(L)ATGC):r(GCAUAUCAG) and d(CT(L)GAT(L)AT(L)GC):r(GCAUAUCAG), along with the unmodified DNA:RNA reference duplex. Increases in the melting temperatures of +9.6 degrees C and +8.1 degrees C per modification relative to the unmodified duplex were observed for these two LNA:RNA sequences. The three duplexes all adopt right-handed helix conformations and form normal Watson-Crick base pairs with all the bases in the anti conformation. Sugar conformations were determined from measurements of scalar coupling constants in the sugar rings and distance information derived from 1H-1H NOE measurements; all the sugars in the RNA strands of the three duplexes adopt an N-type conformation (A-type structure), whereas the sugars in the DNA strands change from an equilibrium between S- and N-type conformations in the unmodified duplex towards more of the N-type conformation when modified nucleotides are introduced. The presence of three modified T(L) nucleotides induces drastic conformational shifts of the remaining unmodified nucleotides of the DNA strand, changing all the sugar conformations except those of the terminal sugars to the N type. The CD spectra of the three duplexes confirm the structural changes described above. On the basis of the results reported herein, we suggest that the observed conformational changes can be used to tune LNA:RNA duplexes into substrates for RNase H: Partly modified LNA:RNA duplexes may adopt a duplex structure between the standard A and B types, thereby making the RNA strand amenable to RNase H-mediated degradation.
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(as it appears on PubMed at http://www.pubmed.gov), where 10985717 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10985717}}
==About this Structure==
==About this Structure==
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1HG9 is a [[Single protein]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG9 OCA].
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1HG9 is a [[Single protein]] structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HG9 OCA].
==Reference==
==Reference==
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[[Category: Rna]]
[[Category: Rna]]
[[Category: Rnase h]]
[[Category: Rnase h]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:49:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:08:56 2008''

Revision as of 05:08, 1 July 2008

Image:1hg9.png

Template:STRUCTURE 1hg9

SOLUTION STRUCTURE OF DNA:RNA HYBRID

Template:ABSTRACT PUBMED 10985717

About this Structure

1HG9 is a Single protein structure. Full experimental information is available from OCA.

Reference

Structural studies of LNA:RNA duplexes by NMR: conformations and implications for RNase H activity., Bondensgaard K, Petersen M, Singh SK, Rajwanshi VK, Kumar R, Wengel J, Jacobsen JP, Chemistry. 2000 Aug 4;6(15):2687-95. PMID:10985717

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