1hji

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[[Image:1hji.gif|left|200px]]
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{{Seed}}
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[[Image:1hji.png|left|200px]]
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{{STRUCTURE_1hji| PDB=1hji | SCENE= }}
{{STRUCTURE_1hji| PDB=1hji | SCENE= }}
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'''BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX'''
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===BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX===
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==Overview==
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Nun protein from coliphage HK022 binds to phage boxB RNA and functions, in contrast to phage lambda N protein, as a transcriptional terminator. The basic Nun-(10-44) peptide contains the boxB RNA binding arginine rich motif, ARM. The peptide binds boxB RNA and competes with the phage lambda ARM peptide N-(1-36) as indicated by nuclear magnetic resonance (NMR) spectroscopy titrations. In two-dimensional nuclear Overhauser enhancement spectroscopy experiments boxB RNA in complex with Nun-(20-44) exhibits the same pattern of resonances as it does in complex with N peptides containing the ARM, and we could show that Nun-(20-44) forms a bent alpha-helix upon binding to the boxB RNA. The structure of the boxB RNA-bound Nun-(20-44) was determined on the basis of 191 intra- and 30 intermolecular distance restraints. Ser-24 is anchored to the lower RNA stem, and stacking of Tyr-39 and A7 is clearly experimentally indicated. Arg-28 shows numerous contacts to the RNA stem. Leu-22, Ile-30, Trp-33, Ile-37, and Leu-41 form a hydrophobic surface, which could be a recognition site for additional host factors such as NusG. Such a hydrophobic surface area is not present in N-(1-36) bound to boxB RNA.
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The line below this paragraph, {{ABSTRACT_PUBMED_11356847}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11356847 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11356847}}
==About this Structure==
==About this Structure==
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1HJI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk022 Enterobacteria phage hk022]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJI OCA].
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1HJI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Enterobacteria_phage_hk022 Enterobacteria phage hk022]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJI OCA].
==Reference==
==Reference==
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[[Category: Protein/rna]]
[[Category: Protein/rna]]
[[Category: Termination]]
[[Category: Termination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:54:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:16:18 2008''

Revision as of 05:16, 1 July 2008

Image:1hji.png

Template:STRUCTURE 1hji

BACTERIOPHAGE HK022 NUN-PROTEIN-NUTBOXB-RNA COMPLEX

Template:ABSTRACT PUBMED 11356847

About this Structure

1HJI is a Protein complex structure of sequences from Enterobacteria phage hk022. Full experimental information is available from OCA.

Reference

The structure of the coliphage HK022 Nun protein-lambda-phage boxB RNA complex. Implications for the mechanism of transcription termination., Faber C, Scharpf M, Becker T, Sticht H, Rosch P, J Biol Chem. 2001 Aug 24;276(34):32064-70. Epub 2001 May 16. PMID:11356847

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