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1hjr

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[[Image:1hjr.jpg|left|200px]]
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{{STRUCTURE_1hjr| PDB=1hjr | SCENE= }}
{{STRUCTURE_1hjr| PDB=1hjr | SCENE= }}
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'''ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI'''
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===ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI===
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==Overview==
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The crystal structure of the RuvC protein, a Holliday junction resolvase from E. coli, has been determined at 2.5 A resolution. The enzyme forms a dimer of 19 kDa subunits related by a dyad axis. Together with results from extensive mutational analyses, the refined structure reveals that the catalytic center, comprising four acidic residues, lies at the bottom of a cleft that nicely fits a DNA duplex. The structural features of the dimer, with a 30 A spacing between the two catalytic centers, provide a substantially defined image of the Holliday junction architecture. The folding topology in the vicinity of the catalytic site exhibits a striking similarity to that of RNAase H1 from E. coli.
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(as it appears on PubMed at http://www.pubmed.gov), where 7923356 is the PubMed ID number.
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{{ABSTRACT_PUBMED_7923356}}
==About this Structure==
==About this Structure==
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[[Category: Vassylyev, D G.]]
[[Category: Vassylyev, D G.]]
[[Category: Site-specific recombinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:55:08 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:17:02 2008''

Revision as of 05:17, 1 July 2008

Image:1hjr.png

Template:STRUCTURE 1hjr

ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI

Template:ABSTRACT PUBMED 7923356

About this Structure

1HJR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli., Ariyoshi M, Vassylyev DG, Iwasaki H, Nakamura H, Shinagawa H, Morikawa K, Cell. 1994 Sep 23;78(6):1063-72. PMID:7923356

Page seeded by OCA on Tue Jul 1 08:17:02 2008

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