1hlg

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{{STRUCTURE_1hlg| PDB=1hlg | SCENE= }}
{{STRUCTURE_1hlg| PDB=1hlg | SCENE= }}
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'''CRYSTAL STRUCTURE OF HUMAN GASTRIC LIPASE'''
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===CRYSTAL STRUCTURE OF HUMAN GASTRIC LIPASE===
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==Overview==
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Fat digestion in humans requires not only the classical pancreatic lipase but also gastric lipase, which is stable and active despite the highly acidic stomach environment. We report here the structure of recombinant human gastric lipase at 3.0-A resolution, the first structure to be described within the mammalian acid lipase family. This globular enzyme (379 residues) consists of a core domain belonging to the alpha/beta hydrolase-fold family and a "cap" domain, which is analogous to that present in serine carboxypeptidases. It possesses a classical catalytic triad (Ser-153, His-353, Asp-324) and an oxyanion hole (NH groups of Gln-154 and Leu-67). Four N-glycosylation sites were identified on the electron density maps. The catalytic serine is deeply buried under a segment consisting of 30 residues, which can be defined as a lid and belonging to the cap domain. The displacement of the lid is necessary for the substrates to have access to Ser-153. A phosphonate inhibitor was positioned in the active site that clearly suggests the location of the hydrophobic substrate binding site. The lysosomal acid lipase was modeled by homology, and possible explanations for some previously reported mutations leading to the cholesterol ester storage disease are given based on the present model.
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(as it appears on PubMed at http://www.pubmed.gov), where 10358049 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10358049}}
==About this Structure==
==About this Structure==
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[[Category: Verger, R.]]
[[Category: Verger, R.]]
[[Category: Lipase]]
[[Category: Lipase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:58:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:21:40 2008''

Revision as of 05:21, 1 July 2008

Image:1hlg.png

Template:STRUCTURE 1hlg

CRYSTAL STRUCTURE OF HUMAN GASTRIC LIPASE

Template:ABSTRACT PUBMED 10358049

About this Structure

1HLG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human gastric lipase and model of lysosomal acid lipase, two lipolytic enzymes of medical interest., Roussel A, Canaan S, Egloff MP, Riviere M, Dupuis L, Verger R, Cambillau C, J Biol Chem. 1999 Jun 11;274(24):16995-7002. PMID:10358049

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