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| | {{STRUCTURE_1hlx| PDB=1hlx | SCENE= }} | | {{STRUCTURE_1hlx| PDB=1hlx | SCENE= }} |
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| - | '''P1 HELIX NUCLEIC ACIDS (DNA/RNA) RIBONUCLEIC ACID'''
| + | ===P1 HELIX NUCLEIC ACIDS (DNA/RNA) RIBONUCLEIC ACID=== |
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| - | ==Overview==
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| - | The upstream cleavage site of group I self-splicing introns is identified by an absolutely conserved U.G base-pair within a double helix. Mutant introns with a wobble C.A substitute are catalytically active, but all other combinations of nucleotides at these positions abolish splicing, suggesting that an unusual RNA structure generated by the wobble pair is recognized by the catalytic intron core. The solution structure of a 20-mer oligonucleotide containing a UUCG tetraloop hairpin and a U.G wobble pair within a double helix was determined by NMR spectroscopy without any assumptions on RNA conformation. Isotopically (15N/13C)-labelled RNA was used to collect an unusually large number of experimental constraints (703 in total, corresponding to approximately 35 constraints per nucleotide) leading to the determination of a structure with very high precision (overall root-mean-square-deviation (rmsd) between 20 converged structures 1.22 A, local rmsd 0.6 A for the tetraloop and 0.85 A for the stem). Analysis of the double helical structure at the conserved U.G wobble pair reveals local distortions from the regular A-form pattern, that may constitute the characteristic feature of U.G wobble pair recognized by the group I intron core and by amino acyl tRNA synthetases. Re-examination of the previously determined tetraloop structure reveals a novel U.G base-pair with a syn guanosine and hydrogen bonding contacts involving both base protons and a sugar 2'-OH. This explains the great stability of RNA UUCG loops when compared with DNA loops of identical sequence, and is one of the first NMR observations of RNA 2'-OH resonances. | + | The line below this paragraph, {{ABSTRACT_PUBMED_7608979}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 7608979 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_7608979}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HLX OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HLX OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Uucg tetraloop hairpin]] | | [[Category: Uucg tetraloop hairpin]] |
| | [[Category: Wobble pair]] | | [[Category: Wobble pair]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 18:59:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:22:37 2008'' |
Revision as of 05:22, 1 July 2008
Template:STRUCTURE 1hlx
P1 HELIX NUCLEIC ACIDS (DNA/RNA) RIBONUCLEIC ACID
Template:ABSTRACT PUBMED 7608979
About this Structure
Full experimental information is available from OCA.
Reference
Structure of the P1 helix from group I self-splicing introns., Allain FH, Varani G, J Mol Biol. 1995 Jul 14;250(3):333-53. PMID:7608979
Page seeded by OCA on Tue Jul 1 08:22:37 2008
