1hn3

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{{STRUCTURE_1hn3| PDB=1hn3 | SCENE= }}
{{STRUCTURE_1hn3| PDB=1hn3 | SCENE= }}
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'''SOLUTION STRUCTURE OF THE N-TERMINAL 37 AMINO ACIDS OF THE MOUSE ARF TUMOR SUPPRESSOR PROTEIN'''
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===SOLUTION STRUCTURE OF THE N-TERMINAL 37 AMINO ACIDS OF THE MOUSE ARF TUMOR SUPPRESSOR PROTEIN===
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==Overview==
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Arf is a tumor suppressor that regulates p53 function and is a frequent target for loss in human cancers. Through two novel mechanisms, Arf inhibits the oncoprotein Hdm2, a negative regulator of p53. (1) Arf inhibits the E3 ubiquitin ligase activity of Hdm2 that leads to p53 degradation, and (2) Arf sequesters Hdm2 within nucleoli. These activities of Arf promote p53-mediated cell cycle arrest and apoptosis. Fundamental to these processes are interactions between Arf and Hdm2. Here we show that a peptide containing the 37 N-terminal amino acids of mouse Arf (mArfN37) localizes to nucleoli, sequesters Hdm2 within nucleoli, and causes cell cycle arrest. Circular dichroism and NMR spectroscopy show that mArfN37 is largely unstructured under aqueous conditions; however, the peptide adopts two alpha-helices (helix 1, residues 4-14; and helix 2, residues 20-29) in 2,2,2-trifluoroethanol (TFE). Each helix contains an amino acid motif that is repeated twice in mArfN37, once in each helix. The two helices, however, do not interact but are connected by an apparently flexible linker. The repeated motif contains Arg residues spaced by a hydrophobic segment that may be involved in Hdm2 recognition and binding. The RRPR nucleolar localization signal, contained within residues 31-34, appears to be disordered under all conditions. The identification of two Arf structural modules suggests that short peptides containing the repeated motif may function as Arf mimics and may allow the design of small molecule Arf mimics in the future.
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(as it appears on PubMed at http://www.pubmed.gov), where 11327858 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11327858}}
==About this Structure==
==About this Structure==
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1HN3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HN3 OCA].
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1HN3 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HN3 OCA].
==Reference==
==Reference==
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[[Category: P53]]
[[Category: P53]]
[[Category: Tumor suppressor]]
[[Category: Tumor suppressor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:01:50 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:25:15 2008''

Revision as of 05:25, 1 July 2008

Image:1hn3.png

Template:STRUCTURE 1hn3

SOLUTION STRUCTURE OF THE N-TERMINAL 37 AMINO ACIDS OF THE MOUSE ARF TUMOR SUPPRESSOR PROTEIN

Template:ABSTRACT PUBMED 11327858

About this Structure

1HN3 is a Single protein structure of sequence from Mus musculus. Full experimental information is available from OCA.

Reference

Solution structure of the p53 regulatory domain of the p19Arf tumor suppressor protein., DiGiammarino EL, Filippov I, Weber JD, Bothner B, Kriwacki RW, Biochemistry. 2001 Feb 27;40(8):2379-86. PMID:11327858

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