1g7c
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(New page: 200px<br /><applet load="1g7c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g7c, resolution 2.05Å" /> '''YEAST EEF1A:EEF1BA I...)
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Revision as of 13:39, 20 November 2007
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YEAST EEF1A:EEF1BA IN COMPLEX WITH GDPNP
Overview
In the elongation cycle of protein biosynthesis, the nucleotide exchange, factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved, eEF1A-eEF1Balpha structure, we determined the structures of the, eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP, complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha, are consistent with in vivo data. The base, sugar and alpha-phosphate bind, as in other known nucleotide G-protein complexes, whereas the beta- and, gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that, inserts into the Mg2+ binding site of eEF1A is lethal. This together with, the structures emphasizes the essential role of Mg2+ in nucleotide, exchange in the eEF1A-eEF1Balpha complex.
About this Structure
1G7C is a Protein complex structure of sequences from Saccharomyces cerevisiae with 5GP as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex., Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J, Nat Struct Biol. 2001 Jun;8(6):531-4. PMID:11373622
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