1hqi

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[[Image:1hqi.gif|left|200px]]
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{{STRUCTURE_1hqi| PDB=1hqi | SCENE= }}
{{STRUCTURE_1hqi| PDB=1hqi | SCENE= }}
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'''COMPONENT P2 FROM THE MULTICOMPONENT PHENOL HYDROXYLASE, NMR, 11 STRUCTURES'''
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===COMPONENT P2 FROM THE MULTICOMPONENT PHENOL HYDROXYLASE, NMR, 11 STRUCTURES===
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==Overview==
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Phenol hydroxylase from Pseudomonas sp. CF600 is a member of a family of binuclear iron-center-containing multicomponent oxygenases, which catalyzes the conversion of phenol and some of its methyl-substituted derivatives to catechol. In addition to a reductase component which transfers electrons from NADH, optimal turnover of the hydroxylase requires P2, a protein containing 90 amino acids which is readily resolved from the other components. The three-dimensional solution structure of P2 has been solved by 3D heteronuclear NMR spectroscopy. On the basis of 1206 experimental constraints, including 1060 distance constraints obtained from NOEs, 70 phi dihedral angle constraints, 42 psi dihedral angle constraints, and 34 hydrogen bond constraints, a total of 12 converged structures were obtained. The atomic root mean square deviation for the 12 converged structure with respect to the mean coordinates is 2.48 A for the backbone atoms and 3.85 A for all the heavy atoms. This relatively large uncertainty can be ascribed to conformational flexibility and exchange. The molecular structure of P2 is composed of three helices, six antiparallel beta-strands, one beta-hairpin, and some less ordered regions. This is the first structure among the known multicomponent oxygenases. On the basis of the three-dimensional structure of P2, sequence comparisons with similar proteins from other multicomponent oxygenases suggested that all of these proteins may have a conserved structure in the core regions.
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{{ABSTRACT_PUBMED_9012665}}
==About this Structure==
==About this Structure==
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1HQI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQI OCA].
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1HQI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp. Pseudomonas sp.]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HQI OCA].
==Reference==
==Reference==
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[[Category: Oxidoreductase]]
[[Category: Oxidoreductase]]
[[Category: Plasmid]]
[[Category: Plasmid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:07:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:32:35 2008''

Revision as of 05:32, 1 July 2008

Image:1hqi.png

Template:STRUCTURE 1hqi

COMPONENT P2 FROM THE MULTICOMPONENT PHENOL HYDROXYLASE, NMR, 11 STRUCTURES

Template:ABSTRACT PUBMED 9012665

About this Structure

1HQI is a Single protein structure of sequence from Pseudomonas sp.. Full experimental information is available from OCA.

Reference

Solution structure of phenol hydroxylase protein component P2 determined by NMR spectroscopy., Qian H, Edlund U, Powlowski J, Shingler V, Sethson I, Biochemistry. 1997 Jan 21;36(3):495-504. PMID:9012665

Page seeded by OCA on Tue Jul 1 08:32:35 2008

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