1hqj

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{{Seed}}
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{{STRUCTURE_1hqj| PDB=1hqj | SCENE= }}
{{STRUCTURE_1hqj| PDB=1hqj | SCENE= }}
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'''CRYSTAL STRUCTURE OF A DE NOVO DESIGNED TRIMERIC COILED-COIL PEPTIDE'''
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===CRYSTAL STRUCTURE OF A DE NOVO DESIGNED TRIMERIC COILED-COIL PEPTIDE===
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==Overview==
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Because of the simplicity and regularity of the alpha-helical coiled coil relative to other structural motifs, it can be conveniently used to clarify the molecular interactions responsible for protein folding and stability. Here we describe the de novo design and characterization of a two heptad-repeat peptide stabilized by a complex network of inter- and intrahelical salt bridges. Circular dichroism spectroscopy and analytical ultracentrifugation show that this peptide is highly alpha-helical and 100% dimeric tinder physiological buffer conditions. Interestingly, the peptide was shown to switch its oligomerization state from a dimer to a trimer upon increasing ionic strength. The correctness of the rational design principles used here is supported by details of the atomic structure of the peptide deduced from X-ray crystallography. The structure of the peptide shows that it is not a molten globule but assumes a unique, native-like conformation. This de novo peptide thus represents an attractive model system for the design of a molecular recognition system.
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{{ABSTRACT_PUBMED_11206050}}
==About this Structure==
==About this Structure==
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[[Category: De novo design]]
[[Category: De novo design]]
[[Category: Trimer]]
[[Category: Trimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:07:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 08:32:40 2008''

Revision as of 05:32, 1 July 2008

Image:1hqj.png

Template:STRUCTURE 1hqj

CRYSTAL STRUCTURE OF A DE NOVO DESIGNED TRIMERIC COILED-COIL PEPTIDE

Template:ABSTRACT PUBMED 11206050

About this Structure

Full crystallographic information is available from OCA.

Reference

Design of a minimal protein oligomerization domain by a structural approach., Burkhard P, Meier M, Lustig A, Protein Sci. 2000 Dec;9(12):2294-301. PMID:11206050

Page seeded by OCA on Tue Jul 1 08:32:40 2008

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