1g8l
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(New page: 200px<br /><applet load="1g8l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1g8l, resolution 1.95Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 13:41, 20 November 2007
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CRYSTAL STRUCTURE OF ESCHERICHIA COLI MOEA
Overview
BACKGROUND: Molybdenum cofactor (Moco) biosynthesis is an evolutionarily, conserved pathway present in archaea, eubacteria, and eukaryotes. In, humans, genetic abnormalities in the biosynthetic pathway result in Moco, deficiency, which is accompanied by severe neurological symptoms and death, shortly after birth. The Escherichia coli MoeA and MogA proteins are, involved in the final step of Moco biosynthesis: the incorporation of, molybdenum into molybdopterin (MPT), the organic pyranopterin moiety of, Moco. RESULTS: The crystal structure of E. coli MoeA has been refined at 2, A resolution and reveals that the highly elongated MoeA monomer consists, of four clearly separated domains, one of which is structurally related to, MogA, indicating a divergent evolutionary relationship between both, proteins. The active form of MoeA is a dimer, and a putative active site, appears to be localized to a cleft formed between domain II of the first, monomer and domains III and IV of the second monomer. CONCLUSIONS: In, eukaryotes, MogA and MoeA are fused into a single polypeptide chain. The, corresponding mammalian protein gephyrin has also been implicated in the, anchoring of glycinergic receptors to the cytoskeleton at inhibitory, synapses. Based on the structures of MoeA and MogA, gephyrin is surmised, to be a highly organized molecule containing at least five domains. This, multidomain arrangement could provide a structural basis for its, functional diversity. The oligomeric states of MoeA and MogA suggest how, gephyrin could assemble into a hexagonal scaffold at inhibitory synapses.
About this Structure
1G8L is a Single protein structure of sequence from Escherichia coli with GOL as ligand. Full crystallographic information is available from OCA.
Reference
The crystal structure of Escherichia coli MoeA and its relationship to the multifunctional protein gephyrin., Xiang S, Nichols J, Rajagopalan KV, Schindelin H, Structure. 2001 Apr 4;9(4):299-310. PMID:11525167
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