1g92

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Revision as of 13:42, 20 November 2007

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SOLUTION STRUCTURE OF PONERATOXIN

Overview

Poneratoxin is a small neuropeptide found in the venom of the ant, Paraponera clavata. It is stored in the venom reservoir as an inactive, 25-residue peptide. Here we describe both chemically synthesized, poneratoxin and poneratoxin obtained by expression in insect cells. When, expressed in insect cells, poneratoxin was observed attached to cell, membranes. Both synthetic and recombinant ponerotoxins were soluble below, pH 4.5. The structure of synthetic poneratoxin was characterized by, circular dichroism and solved by nuclear magnetic resonance. In an, environment imitating a lipid bilayer, at pH within the range of insect, hemolymph, synthetic poneratoxin has a V shape, with two alpha-helices, connected by a beta-turn. Insect larvae were paralyzed by injection of, either of the purified toxins, with the recombinant one acting faster. The, recombinant toxin-producing baculovirus reduced the average survival time, of the insect host by 25 h compared with unmodified virus. Mass, spectrometry analysis showed that the recombinant toxin has an N-terminal, 21-residue extension, possibly improving its stability and/or stabilizing, the membrane-bound state. The potential use of poneratoxin for the, construction of biological insecticide is discussed.

About this Structure

1G92 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.

Reference

Poneratoxin, a neurotoxin from ant venom. Structure and expression in insect cells and construction of a bio-insecticide., Szolajska E, Poznanski J, Ferber ML, Michalik J, Gout E, Fender P, Bailly I, Dublet B, Chroboczek J, Eur J Biochem. 2004 Jun;271(11):2127-36. PMID:15153103

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