From Proteopedia
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| - | [[Image:1hxs.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1hxs| PDB=1hxs | SCENE= }} | | {{STRUCTURE_1hxs| PDB=1hxs | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF MAHONEY STRAIN OF POLIOVIRUS AT 2.2A RESOLUTION'''
| + | ===CRYSTAL STRUCTURE OF MAHONEY STRAIN OF POLIOVIRUS AT 2.2A RESOLUTION=== |
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| - | ==Overview==
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| - | A genetic algorithm-based computational method for the ab initio phasing of diffraction data from crystals of symmetric macromolecular structures, such as icosahedral viruses, has been implemented and applied to authentic data from the P1/Mahoney strain of poliovirus. Using only single-wavelength native diffraction data, the method is shown to be able to generate correct phases, and thus electron density, to 3.0 A resolution. Beginning with no advance knowledge of the shape of the virus and only approximate knowledge of its size, the method uses a genetic algorithm to determine coarse, low-resolution (here, 20.5 A) models of the virus that obey the known non-crystallographic symmetry (NCS) constraints. The best scoring of these models are subjected to refinement and NCS-averaging, with subsequent phase extension to high resolution (3.0 A). Initial difficulties in phase extension were overcome by measuring and including all low-resolution terms in the transform. With the low-resolution data included, the method was successful in generating essentially correct phases and electron density to 6.0 A in every one of ten trials from different models identified by the genetic algorithm. Retrospective analysis revealed that these correct high-resolution solutions converged from a range of significantly different low-resolution phase sets (average differences of 59.7 degrees below 24 A). This method represents an efficient way to determine phases for icosahedral viruses, and has the advantage of producing phases free from model bias. It is expected that the method can be extended to other protein systems with high NCS.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11254378}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11254378 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11254378}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Picornavirus]] | | [[Category: Picornavirus]] |
| | [[Category: Poliovirus]] | | [[Category: Poliovirus]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:20:28 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:05:44 2008'' |
Revision as of 07:05, 1 July 2008
Template:STRUCTURE 1hxs
CRYSTAL STRUCTURE OF MAHONEY STRAIN OF POLIOVIRUS AT 2.2A RESOLUTION
Template:ABSTRACT PUBMED 11254378
About this Structure
1HXS is a Protein complex structure of sequences from Human poliovirus 1. Full crystallographic information is available from OCA.
Reference
Ab initio phasing of high-symmetry macromolecular complexes: successful phasing of authentic poliovirus data to 3.0 A resolution., Miller ST, Hogle JM, Filman DJ, J Mol Biol. 2001 Mar 23;307(2):499-512. PMID:11254378
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