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| - | [[Image:1i0u.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:1i0u.png|left|200px]] |
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| | {{STRUCTURE_1i0u| PDB=1i0u | SCENE= }} | | {{STRUCTURE_1i0u| PDB=1i0u | SCENE= }} |
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| - | '''SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF A CONCATEMER OF EGF-HOMOLOGY MODULES OF THE HUMAN LOW DENSITY LIPOPROTEIN RECEPTOR'''
| + | ===SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF A CONCATEMER OF EGF-HOMOLOGY MODULES OF THE HUMAN LOW DENSITY LIPOPROTEIN RECEPTOR=== |
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| - | ==Overview==
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| - | The ligand-binding region of the low-density lipoprotein (LDL) receptor is formed by seven N-terminal, imperfect, cysteine-rich (LB) modules. This segment is followed by an epidermal growth factor precursor homology domain with two N-terminal, tandem, EGF-like modules that are thought to participate in LDL binding and recycling of the endocytosed receptor to the cell surface. EGF-A and the concatemer, EGF-AB, of these modules were expressed in Escherichia coli. Correct protein folding of EGF-A and the concatemer EGF-AB was achieved in the presence or absence of calcium ions, in contrast to the LB modules, which require them for correct folding. Homonuclear and heteronuclear 1H-15N NMR spectroscopy at 17.6 T was used to determine the three-dimensional structure of the concatemer. Both modules are formed by two pairs of short, anti-parallel beta-strands. In the concatemer, these modules have a fixed relative orientation, stabilized by calcium ion-binding and hydrophobic interactions at the interface. 15N longitudinal and transverse relaxation rates, and [1H]-15N heteronuclear NOEs were used to derive a model-free description of the backbone dynamics of the molecule. The concatemer appears relatively rigid, particularly near the calcium ion-binding site at the module interface, with an average generalized order parameter of 0.85+/-0.11. Some mutations causing familial hypercholesterolemia may now be rationalized. Mutations of D41, D43 and E44 in the EGF-B calcium ion-binding region may affect the stability of the linker and thus the orientation of the tandem modules. The diminutive core also provides little structural stabilization, necessitating the presence of disulfide bonds. The structure and dynamics of EGF-AB contrast with the N-terminal LB modules, which require calcium ions both for folding to form the correct disulfide connectivities and for maintenance of the folded structure, and are connected by highly mobile linking peptides. | + | The line below this paragraph, {{ABSTRACT_PUBMED_11478865}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11478865 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11478865}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | 1I0U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0U OCA]. | + | 1I0U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I0U OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Anti-parallel beta strand]] | | [[Category: Anti-parallel beta strand]] |
| | [[Category: Calcium binding site]] | | [[Category: Calcium binding site]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:26:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:14:39 2008'' |
Revision as of 07:14, 1 July 2008
Template:STRUCTURE 1i0u
SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF A CONCATEMER OF EGF-HOMOLOGY MODULES OF THE HUMAN LOW DENSITY LIPOPROTEIN RECEPTOR
Template:ABSTRACT PUBMED 11478865
About this Structure
1I0U is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.
Reference
NMR structure and backbone dynamics of a concatemer of epidermal growth factor homology modules of the human low-density lipoprotein receptor., Kurniawan ND, Aliabadizadeh K, Brereton IM, Kroon PA, Smith R, J Mol Biol. 2001 Aug 10;311(2):341-56. PMID:11478865
Page seeded by OCA on Tue Jul 1 10:14:39 2008
