1i44

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{{STRUCTURE_1i44| PDB=1i44 | SCENE= }}
{{STRUCTURE_1i44| PDB=1i44 | SCENE= }}
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'''CRYSTALLOGRAPHIC STUDIES OF AN ACTIVATION LOOP MUTANT OF THE INSULIN RECEPTOR TYROSINE KINASE'''
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===CRYSTALLOGRAPHIC STUDIES OF AN ACTIVATION LOOP MUTANT OF THE INSULIN RECEPTOR TYROSINE KINASE===
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==Overview==
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The tyrosine kinase domain of the insulin receptor is subject to autoinhibition in the unphosphorylated basal state via steric interactions involving the activation loop. A mutation in the activation loop designed to relieve autoinhibition, Asp-1161 --&gt; Ala, substantially increases the ability of the unphosphorylated kinase to bind ATP. The crystal structure of this mutant in complex with an ATP analog has been determined at 2.4-A resolution. The structure shows that the active site is unobstructed, but the end of the activation loop is disordered and therefore the binding site for peptide substrates is not fully formed. In addition, Phe-1151 of the protein kinase-conserved DFG motif, at the beginning of the activation loop, hinders closure of the catalytic cleft and proper positioning of alpha-helix C for catalysis. These results, together with viscometric kinetic measurements, suggest that peptide substrate binding induces a reconfiguration of the unphosphorylated activation loop prior to the catalytic step. The crystallographic and solution studies provide new insights into the mechanism by which the activation loop controls phosphoryl transfer as catalyzed by the insulin receptor.
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(as it appears on PubMed at http://www.pubmed.gov), where 11124964 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11124964}}
==About this Structure==
==About this Structure==
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[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]
[[Category: Protein tyrosine kinase]]
[[Category: Protein tyrosine kinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:23:15 2008''

Revision as of 07:23, 1 July 2008

Image:1i44.png

Template:STRUCTURE 1i44

CRYSTALLOGRAPHIC STUDIES OF AN ACTIVATION LOOP MUTANT OF THE INSULIN RECEPTOR TYROSINE KINASE

Template:ABSTRACT PUBMED 11124964

About this Structure

1I44 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystallographic and solution studies of an activation loop mutant of the insulin receptor tyrosine kinase: insights into kinase mechanism., Till JH, Ablooglu AJ, Frankel M, Bishop SM, Kohanski RA, Hubbard SR, J Biol Chem. 2001 Mar 30;276(13):10049-55. Epub 2000 Dec 21. PMID:11124964

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