1i4v

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[[Image:1i4v.gif|left|200px]]
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[[Image:1i4v.png|left|200px]]
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{{STRUCTURE_1i4v| PDB=1i4v | SCENE= }}
{{STRUCTURE_1i4v| PDB=1i4v | SCENE= }}
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'''SOLUTION STRUCTURE OF THE UMUD' HOMODIMER'''
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===SOLUTION STRUCTURE OF THE UMUD' HOMODIMER===
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==Overview==
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During the SOS response of Escherichia coli to DNA damage, the umuDC operon is induced, producing the trimeric protein complexes UmuD2C, a DNA damage checkpoint effector, and UmuD'2C (DNA polymerase V), which carries out translesion synthesis, the basis of 'SOS mutagenesis'. UmuD'2, the homodimeric component of DNA pol V, is produced from UmuD by RecA-facilitated self-cleavage, which removes the 24 N-terminal residues of UmuD. We report the solution structure of UmuD'2 (PDB ID 1I4V) and interactions within UmuD'-UmuD, a heterodimer inactive in translesion synthesis. The overall shape of UmuD'2 in solution differs substantially from the previously reported crystal structure, even though the topologies of the two structures are quite similar. Most significantly, the active site residues S60 and K97 do not point directly at one another in solution as they do in the crystal, suggesting that self-cleavage of UmuD might require RecA to assemble the active site. Structural differences between UmuD'2 and UmuD'- UmuD suggest that UmuD'2C and UmuD2C might achieve their different biological activities through distinct interactions with RecA and DNA pol III.
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The line below this paragraph, {{ABSTRACT_PUBMED_11483531}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11483531 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11483531}}
==About this Structure==
==About this Structure==
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1I4V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4V OCA].
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1I4V is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4V OCA].
==Reference==
==Reference==
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[[Category: Sos mutagenesis]]
[[Category: Sos mutagenesis]]
[[Category: Sos response]]
[[Category: Sos response]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:34:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:25:21 2008''

Revision as of 07:25, 1 July 2008

Image:1i4v.png

Template:STRUCTURE 1i4v

SOLUTION STRUCTURE OF THE UMUD' HOMODIMER

Template:ABSTRACT PUBMED 11483531

About this Structure

1I4V is a Single protein structure of sequence from Escherichia coli. Full experimental information is available from OCA.

Reference

Converting a DNA damage checkpoint effector (UmuD2C) into a lesion bypass polymerase (UmuD'2C)., Ferentz AE, Walker GC, Wagner G, EMBO J. 2001 Aug 1;20(15):4287-98. PMID:11483531

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