1i50

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[[Image:1i50.gif|left|200px]]
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{{STRUCTURE_1i50| PDB=1i50 | SCENE= }}
{{STRUCTURE_1i50| PDB=1i50 | SCENE= }}
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'''RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION'''
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===RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION===
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==Overview==
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Structures of a 10-subunit yeast RNA polymerase II have been derived from two crystal forms at 2.8 and 3.1 angstrom resolution. Comparison of the structures reveals a division of the polymerase into four mobile modules, including a clamp, shown previously to swing over the active center. In the 2.8 angstrom structure, the clamp is in an open state, allowing entry of straight promoter DNA for the initiation of transcription. Three loops extending from the clamp may play roles in RNA unwinding and DNA rewinding during transcription. A 2.8 angstrom difference Fourier map reveals two metal ions at the active site, one persistently bound and the other possibly exchangeable during RNA synthesis. The results also provide evidence for RNA exit in the vicinity of the carboxyl-terminal repeat domain, coupling synthesis to RNA processing by enzymes bound to this domain.
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The line below this paragraph, {{ABSTRACT_PUBMED_11313498}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11313498 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11313498}}
==About this Structure==
==About this Structure==
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Zinc motif]]
[[Category: Zinc motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:34:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:25:41 2008''

Revision as of 07:25, 1 July 2008

Image:1i50.png

Template:STRUCTURE 1i50

RNA POLYMERASE II CRYSTAL FORM II AT 2.8 A RESOLUTION

Template:ABSTRACT PUBMED 11313498

About this Structure

1I50 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution., Cramer P, Bushnell DA, Kornberg RD, Science. 2001 Jun 8;292(5523):1863-76. Epub 2001 Apr 19. PMID:11313498

Page seeded by OCA on Tue Jul 1 10:25:41 2008

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