1gcg
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(New page: 200px<br /><applet load="1gcg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gcg, resolution 1.9Å" /> '''THE 1.9 ANGSTROMS X-R...)
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Revision as of 13:49, 20 November 2007
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THE 1.9 ANGSTROMS X-RAY STRUCTURE OF A CLOSED UNLIGANDED FORM OF THE PERIPLASMIC GLUCOSE(SLASH)GALACTOSE RECEPTOR FROM SALMONELLA TYPHIMURIUM
Overview
The three-dimensional structure of a ligand-free closed form of the, glucose/galactose binding protein from Salmonella typhimurium has been, determined at a resolution of 1.9 A. The crystallographic R-factor for the, refined structure is 17.9%. The model contains all the atoms of the 309, residues of the protein sequence, a calcium ion, and 174 water molecules., The root mean square (r.m.s.) deviations for the whole molecule are: 0.010, A for bond lengths and 2.44 degrees for bond angles, indicating a good, stereochemistry for the model. This structure shows that the protein is, able to close in the absence of ligand, adopting a conformation similar to, the liganded form but slightly more open. Water molecules satisfy the, hydrogen bonding ability of the hydrophilic side chains of the binding, site in a manner which is reminiscent of the sugars' hydrogen-bonding, patterns. Since packing forces are weak, the crystallization event is, unlikely to trigger a change from an open to a closed conformation., Instead, the latter must be one of the species in equilibrium in solution, which is selected by packing in the crystal lattice.
About this Structure
1GCG is a Single protein structure of sequence from Salmonella typhimurium with CA as ligand. Full crystallographic information is available from OCA.
Reference
The 1.9 A x-ray structure of a closed unliganded form of the periplasmic glucose/galactose receptor from Salmonella typhimurium., Flocco MM, Mowbray SL, J Biol Chem. 1994 Mar 25;269(12):8931-6. PMID:8132630
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