This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1i7k

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1i7k.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1i7k.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1i7k| PDB=1i7k | SCENE= }}
{{STRUCTURE_1i7k| PDB=1i7k | SCENE= }}
-
'''CRYSTAL STRUCTURE OF HUMAN MITOTIC-SPECIFIC UBIQUITIN-CONJUGATING ENZYME, UBCH10'''
+
===CRYSTAL STRUCTURE OF HUMAN MITOTIC-SPECIFIC UBIQUITIN-CONJUGATING ENZYME, UBCH10===
-
==Overview==
+
<!--
-
Cell cycle progression is controlled at several different junctures by the targeted destruction of cell cycle regulatory proteins. These carefully orchestrated events include the destruction of the securin protein to permit entry into anaphase, and the destruction of cyclin B to permit exit from mitosis. These destruction events are mediated by the ubiquitin/proteasome system. The human ubiquitin-conjugating enzyme, UbcH10, is an essential mediator of the mitotic destruction events. We report here the 1.95-A crystal structure of a mutant UbcH10, in which the active site cysteine has been replaced with a serine. Functional analysis indicates that the mutant is active in accepting ubiquitin, although not as efficiently as wild-type. Examination of the crystal structure reveals that the NH2-terminal extension in UbcH10 is disordered and that a conserved 3(10)-helix places a lysine residue near the active site. Analysis of relevant mutants demonstrates that for ubiquitin-adduct formation the presence or absence of the NH2-terminal extension has little effect, whereas the lysine residue near the active site has significant effect. The structure provides additional insight into UbcH10 function including possible sites of interaction with the anaphase promoting complex/cyclosome and the disposition of a putative destruction box motif in the structure.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11927573}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11927573 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11927573}}
==About this Structure==
==About this Structure==
Line 26: Line 30:
[[Category: Lin, Y.]]
[[Category: Lin, Y.]]
[[Category: Ubiquitin conjugating enzyme]]
[[Category: Ubiquitin conjugating enzyme]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:40:22 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:32:28 2008''

Revision as of 07:32, 1 July 2008

Image:1i7k.png

Template:STRUCTURE 1i7k

CRYSTAL STRUCTURE OF HUMAN MITOTIC-SPECIFIC UBIQUITIN-CONJUGATING ENZYME, UBCH10

Template:ABSTRACT PUBMED 11927573

About this Structure

1I7K is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural and functional analysis of the human mitotic-specific ubiquitin-conjugating enzyme, UbcH10., Lin Y, Hwang WC, Basavappa R, J Biol Chem. 2002 Jun 14;277(24):21913-21. Epub 2002 Apr 1. PMID:11927573

Page seeded by OCA on Tue Jul 1 10:32:28 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1i7k"
Personal tools