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1i8d

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[[Image:1i8d.gif|left|200px]]
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{{STRUCTURE_1i8d| PDB=1i8d | SCENE= }}
{{STRUCTURE_1i8d| PDB=1i8d | SCENE= }}
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'''CRYSTAL STRUCTURE OF RIBOFLAVIN SYNTHASE'''
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===CRYSTAL STRUCTURE OF RIBOFLAVIN SYNTHASE===
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==Overview==
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BACKGROUND: Riboflavin synthase catalyzes the dismutation of two molecules of 6,7-dimethyl-8-(1'-D-ribityl)-lumazine to yield riboflavin and 4-ribitylamino-5-amino-2,6-dihydroxypyrimidine. The homotrimer of 23 kDa subunits has no cofactor requirements for catalysis. The enzyme is nonexistent in humans and is an attractive target for antimicrobial agents of organisms whose pathogenicity depends on their ability to biosynthesize riboflavin. RESULTS: The first three-dimensional structure of the enzyme was determined at 2.0 A resolution using the multiwavelength anomalous diffraction (MAD) method on the Escherichia coli protein containing selenomethionine residues. The homotrimer consists of an asymmetric assembly of monomers, each of which comprises two similar beta barrels and a C-terminal alpha helix. The similar beta barrels within the monomer confirm a prediction of pseudo two-fold symmetry that is inferred from the sequence similarity between the two halves of the protein. The beta barrels closely resemble folds found in phthalate dioxygenase reductase and other flavoproteins. CONCLUSIONS: The three active sites of the trimer are proposed to lie between pairs of monomers in which residues conserved among species reside, including two Asp-His-Ser triads and dyads of Cys-Ser and His-Thr. The proposed active sites are located where FMN (an analog of riboflavin) is modeled from an overlay of the beta barrels of phthalate dioxygenase reductase and riboflavin synthase. In the trimer, one active site is formed, and the other two active sites are wide open and exposed to solvent. The nature of the trimer configuration suggests that only one active site can be formed and be catalytically competent at a time.
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The line below this paragraph, {{ABSTRACT_PUBMED_11377200}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11377200 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11377200}}
==About this Structure==
==About this Structure==
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[[Category: Riboflavin synthase]]
[[Category: Riboflavin synthase]]
[[Category: Structure-based design]]
[[Category: Structure-based design]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:42:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:34:39 2008''

Revision as of 07:34, 1 July 2008

Image:1i8d.png

Template:STRUCTURE 1i8d

CRYSTAL STRUCTURE OF RIBOFLAVIN SYNTHASE

Template:ABSTRACT PUBMED 11377200

About this Structure

1I8D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of riboflavin synthase., Liao DI, Wawrzak Z, Calabrese JC, Viitanen PV, Jordan DB, Structure. 2001 May 9;9(5):399-408. PMID:11377200

Page seeded by OCA on Tue Jul 1 10:34:39 2008

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