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1gdt
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(New page: 200px<br /><applet load="1gdt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gdt, resolution 3.000Å" /> '''CRYSTAL STRUCTURE O...)
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Revision as of 13:51, 20 November 2007
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CRYSTAL STRUCTURE OF A SITE-SPECIFIC RECOMBINASE, GAMMA-DELTA RESOLVASE COMPLEXED WITH A 34 BP CLEAVAGE SITE
Overview
The structure of gamma delta resolvase complexed with a 34 bp substrate, DNA has been determined at 3.0 A resolution. The DNA is sharply bent by 60, degrees toward the major groove and away from the resolvase catalytic, domains at the recombination crossover point. The C-terminal one third of, resolvase, which was disordered in the absence of DNA, forms an arm and a, 3-helix DNA-binding domain on the opposite side of the DNA from the, N-terminal domain. The arms wrap around the minor groove of the central 16, bp, and the DNA-binding domains interact with the major grooves near the, outer boundaries of the binding site. The resolvase dimer is asymmetric, particularly in the arm region, implying a conformational adaptability, that may be important for resolvase binding to different DNA sites in the, synaptosome. It also raises the possibility of a sequential single-strand, cleavage mechanism.
About this Structure
1GDT is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of the site-specific recombinase gamma delta resolvase complexed with a 34 bp cleavage site., Yang W, Steitz TA, Cell. 1995 Jul 28;82(2):193-207. PMID:7628011
Page seeded by OCA on Tue Nov 20 15:58:15 2007
