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1id5

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[[Image:1id5.jpg|left|200px]]
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{{STRUCTURE_1id5| PDB=1id5 | SCENE= }}
{{STRUCTURE_1id5| PDB=1id5 | SCENE= }}
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'''CRYSTAL STRUCTURE OF BOVINE THROMBIN COMPLEX WITH PROTEASE INHIBITOR ECOTIN'''
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===CRYSTAL STRUCTURE OF BOVINE THROMBIN COMPLEX WITH PROTEASE INHIBITOR ECOTIN===
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==Overview==
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The protease inhibitor ecotin fails to inhibit thrombin despite its broad specificity against serine proteases. A point mutation (M84R) in ecotin results in a 1.5 nM affinity for thrombin, 10(4) times stronger than that of wild-type ecotin. The crystal structure of bovine thrombin is determined in complex with ecotin M84R mutant at 2.5 A resolution. Surface loops surrounding the active site cleft of thrombin have undergone significant structural changes to permit inhibitor binding. Particularly, the insertion loops at residues 60 and 148 in thrombin, which likely mediate the interactions with macromolecules, are displaced when the complex forms. Thrombin and ecotin M84R interact in two distinct surfaces. The loop at residue 99 and the C-terminus of thrombin contact ecotin through mixed polar and nonpolar interactions. The active site of thrombin is filled with eight consecutive amino acids of ecotin and demonstrates thrombin's preference for specific features that are compatible with the thrombin cleavage site: negatively charged-Pro-Val-X-Pro-Arg-hydrophobic-positively charged (P1 Arg is in bold letters). The preference for a Val at P4 is clearly defined. The insertion at residue 60 may further affect substrate binding by moving its adjacent loops that are part of the substrate recognition sites.
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The line below this paragraph, {{ABSTRACT_PUBMED_11513582}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11513582 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11513582}}
==About this Structure==
==About this Structure==
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[[Category: Ecotin m84r]]
[[Category: Ecotin m84r]]
[[Category: Thrombin]]
[[Category: Thrombin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:51:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:47:18 2008''

Revision as of 07:47, 1 July 2008

Image:1id5.png

Template:STRUCTURE 1id5

CRYSTAL STRUCTURE OF BOVINE THROMBIN COMPLEX WITH PROTEASE INHIBITOR ECOTIN

Template:ABSTRACT PUBMED 11513582

About this Structure

1ID5 is a Protein complex structure of sequences from Bos taurus and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of thrombin-ecotin reveals conformational changes and extended interactions., Wang SX, Esmon CT, Fletterick RJ, Biochemistry. 2001 Aug 28;40(34):10038-46. PMID:11513582

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