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1idl

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{{STRUCTURE_1idl| PDB=1idl | SCENE= }}
{{STRUCTURE_1idl| PDB=1idl | SCENE= }}
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'''THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN'''
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===THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN===
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==Overview==
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The region encompassing residues 181-98 on the alpha1 subunit of the muscle-type nicotinic acetylcholine receptor forms a major determinant for the binding of alpha-neurotoxins. We have prepared an (15)N-enriched 18-amino acid peptide corresponding to the sequence in this region to facilitate structural elucidation by multidimensional NMR. Our aim was to determine the structural basis for the high affinity, stoichiometric complex formed between this cognate peptide and alpha-bungarotoxin, a long alpha-neurotoxin. Resonances in the complex were assigned through heteronuclear and homonuclear NMR experiments, and the resulting interproton distance constraints were used to generate ensemble structures of the complex. Thr(8), Pro(10), Lys(38), Val(39), Val(40), and Pro(69) in alpha-bungarotoxin and Tyr(189), Tyr(190), Thr(191), Cys(192), Asp(195), and Thr(196) in the peptide participate in major intermolecular contacts. A comparison of the free and bound alpha-bungarotoxin structures reveals significant conformational rearrangements in flexible regions of alpha-bungarotoxin, mainly loops I, II, and the C-terminal tail. Furthermore, several of the calculated structures suggest that cation-pi interactions may be involved in binding. The root mean square deviation of the polypeptide backbone in the complex is 2.07 A. This structure provides, to date, the highest resolution description of the contacts between a prototypic alpha-neurotoxin and its cognate recognition sequence.
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(as it appears on PubMed at http://www.pubmed.gov), where 11312275 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11312275}}
==About this Structure==
==About this Structure==
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1IDL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA].
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1IDL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_multicinctus Bungarus multicinctus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IDL OCA].
==Reference==
==Reference==
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[[Category: Alpha-neurotoxin]]
[[Category: Alpha-neurotoxin]]
[[Category: Nmr solution structure]]
[[Category: Nmr solution structure]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 19:52:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 10:48:20 2008''

Revision as of 07:48, 1 July 2008

Image:1idl.png

Template:STRUCTURE 1idl

THE NMR SOLUTION STRUCTURE OF ALPHA-BUNGAROTOXIN

Template:ABSTRACT PUBMED 11312275

About this Structure

1IDL is a Single protein structure of sequence from Bungarus multicinctus. Full experimental information is available from OCA.

Reference

The solution structure of the complex formed between alpha-bungarotoxin and an 18-mer cognate peptide derived from the alpha 1 subunit of the nicotinic acetylcholine receptor from Torpedo californica., Zeng H, Moise L, Grant MA, Hawrot E, J Biol Chem. 2001 Jun 22;276(25):22930-40. Epub 2001 Apr 18. PMID:11312275

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