1iji

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1iji.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1iji.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1iji| PDB=1iji | SCENE= }}
{{STRUCTURE_1iji| PDB=1iji | SCENE= }}
-
'''Crystal Structure of L-Histidinol Phosphate Aminotransferase with PLP'''
+
===Crystal Structure of L-Histidinol Phosphate Aminotransferase with PLP===
-
==Overview==
+
<!--
-
The biosynthesis of histidine is a central metabolic process in organisms ranging from bacteria to yeast and plants. The seventh step in the synthesis of histidine within eubacteria is carried out by a pyridoxal-5'-phosphate (PLP)-dependent l-histidinol phosphate aminotransferase (HisC, EC 2.6.1.9). Here, we report the crystal structure of l-histidinol phosphate aminotransferase from Escherichia coli, as a complex with pyridoxamine-5'-phosphate (PMP) at 1.5 A resolution, as the internal aldimine with PLP, and in a covalent, tetrahedral complex consisting of PLP and l-histidinol phosphate attached to Lys214, both at 2.2 A resolution. This covalent complex resembles, in structural terms, the gem-diamine intermediate that is formed transiently during conversion of the internal to external aldimine.HisC is a dimeric enzyme with a mass of approximately 80 kDa. Like most PLP-dependent enzymes, each HisC monomer consists of two domains, a larger PLP-binding domain having an alpha/beta/alpha topology, and a smaller domain. An N-terminal arm contributes to the dimerization of the two monomers. The PLP-binding domain of HisC shows weak sequence similarity, but significant structural similarity with the PLP-binding domains of a number of PLP-dependent enzymes. Residues that interact with the PLP cofactor, including Tyr55, Asn157, Asp184, Tyr187, Ser213, Lys214 and Arg222, are conserved in the family of aspartate, tyrosine and histidinol phosphate aminotransferases. The imidazole ring of l-histidinol phosphate is bound, in part, through a hydrogen bond with Tyr110, a residue that is substituted by Phe in the broad substrate specific HisC enzymes from Zymomonas mobilis and Bacillus subtilis.Comparison of the structures of the HisC internal aldimine, the PMP complex and the HisC l-histidinol phosphate complex reveal minimal changes in protein or ligand structure. Proton transfer, required for conversion of the gem-diamine to the external aldimine, does not appear to be limited by the distance between substrate and lysine amino groups. We propose that the tetrahedral complex has resulted from non-productive binding of l-histidinol phosphate soaked into the HisC crystals, resulting in its inability to be converted to the external aldimine at the HisC active site.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11518529}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11518529 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11518529}}
==About this Structure==
==About this Structure==
Line 34: Line 38:
[[Category: Histidine biosynthesis]]
[[Category: Histidine biosynthesis]]
[[Category: Pyridoxal phosphate]]
[[Category: Pyridoxal phosphate]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:04:17 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 12:20:36 2008''

Revision as of 09:20, 1 July 2008

Image:1iji.png

Template:STRUCTURE 1iji

Crystal Structure of L-Histidinol Phosphate Aminotransferase with PLP

Template:ABSTRACT PUBMED 11518529

About this Structure

1IJI is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of histidinol phosphate aminotransferase (HisC) from Escherichia coli, and its covalent complex with pyridoxal-5'-phosphate and l-histidinol phosphate., Sivaraman J, Li Y, Larocque R, Schrag JD, Cygler M, Matte A, J Mol Biol. 2001 Aug 24;311(4):761-76. PMID:11518529

Page seeded by OCA on Tue Jul 1 12:20:36 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1iji"
Personal tools