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| - | [[Image:1ik7.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1ik7| PDB=1ik7 | SCENE= }} | | {{STRUCTURE_1ik7| PDB=1ik7 | SCENE= }} |
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| - | '''Crystal Structure of the Uncomplexed Pelle Death Domain'''
| + | ===Crystal Structure of the Uncomplexed Pelle Death Domain=== |
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| - | ==Overview==
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| - | The death domain (DD) of the protein kinase Pelle adopts a six-helix bundle fold in the crystal structure of the complex with its dimerization partner, Tube-DD. However, in crystals obtained from a solution of 45% 2-methyl-2,4-pentanediol (MPD), the C-terminal half of Pelle-DD folds into a single helix, and the N-terminal half of the molecule is disordered. The helical segment forms an antiparallel dimer with the corresponding helix of a symmetry-related molecule, and together they form extensive lattice interactions similar in number, composition, and buried surface to those in the six-helix bundle of the native fold. Secondary structure analysis by heteronuclear nuclear magnetic resonance spectroscopy (NMR) demonstrates that Pelle-DD adopts a six-helix bundle fold in aqueous solution. The fold is perturbed by MPD, with the largest chemical shift changes in one helix and two loop regions that encompass the Tube-DD binding site. Pelle-DD is stable to urea denaturation with a folding free energy of 7.9 kcal/mol at 25 degrees C but is destabilized, with loss of urea binding sites, in the presence of MPD. The data are consistent with a cosolvent denaturation model in which MPD denatures the N terminus of Pelle-DD but induces the C terminus to form a more compact structure and aggregate. A similar perturbation in vivo might occur at the plasma membrane and could have consequences for Pelle-mediated regulation. Generally, crystallographers should be aware that high concentrations of MPD or related cosolvents can alter the tertiary structure of susceptible proteins. | + | The line below this paragraph, {{ABSTRACT_PUBMED_12177432}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 12177432 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_12177432}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Single helix]] | | [[Category: Single helix]] |
| | [[Category: Structural transition]] | | [[Category: Structural transition]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:05:35 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:21:31 2008'' |
Revision as of 10:21, 1 July 2008
Template:STRUCTURE 1ik7
Crystal Structure of the Uncomplexed Pelle Death Domain
Template:ABSTRACT PUBMED 12177432
About this Structure
1IK7 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Cosolvent-induced transformation of a death domain tertiary structure., Xiao T, Gardner KH, Sprang SR, Proc Natl Acad Sci U S A. 2002 Aug 20;99(17):11151-6. Epub 2002 Aug 12. PMID:12177432
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