1ino

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[[Image:1ino.gif|left|200px]]
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{{STRUCTURE_1ino| PDB=1ino | SCENE= }}
{{STRUCTURE_1ino| PDB=1ino | SCENE= }}
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'''RECOMBINANT INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI'''
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===RECOMBINANT INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI===
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==Overview==
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An E. coli inorganic pyrophosphatase overproducer and a method for a large-scale production of the homogeneous enzyme are described. The inorganic pyrophosphatase was crystallized in the form containing one subunit of a homohexameric molecule per asymmetric unit: space group R32, a = 110.4 A, c = 76.8 A. The electron density map to 2.5 A resolution phased with Eu- and Hg-derivatives (figure of merit, &lt;m&gt; = 0.51) was improved by the solvent flattening procedure (&lt;m&gt; = 0.77). The course of the polypeptide chain and the secondary structure elements, intersubunit contacts and positions of the active sites were characterized. Homology with S. cerevisiae inorganic pyrophosphatase structure was found.
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(as it appears on PubMed at http://www.pubmed.gov), where 8034059 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8034059}}
==About this Structure==
==About this Structure==
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[[Category: Metal binding]]
[[Category: Metal binding]]
[[Category: Mn2+ ion]]
[[Category: Mn2+ ion]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:11:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:37:17 2008''

Revision as of 10:37, 1 July 2008

Image:1ino.png

Template:STRUCTURE 1ino

RECOMBINANT INORGANIC PYROPHOSPHATASE FROM ESCHERICHIA COLI

Template:ABSTRACT PUBMED 8034059

About this Structure

1INO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

X-ray crystallographic studies of recombinant inorganic pyrophosphatase from Escherichia coli., Oganessyan VYu, Kurilova SA, Vorobyeva NN, Nazarova TI, Popov AN, Lebedev AA, Avaeva SM, Harutyunyan EH, FEBS Lett. 1994 Jul 18;348(3):301-4. PMID:8034059

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