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1ioo

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{{STRUCTURE_1ioo| PDB=1ioo | SCENE= }}
{{STRUCTURE_1ioo| PDB=1ioo | SCENE= }}
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'''CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE'''
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===CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE===
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==Overview==
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The crystal structure of Nicotiana alata (ornamental tobacco) S(F11)-RNase, an S-allelic glycoprotein associated with gametophytic self-incompatibility, was determined by X-ray diffraction at 1.55 A resolution. The protein has a tertiary structure typical of members of the RNase T(2) family as it consists of a variant of the (alpha+beta) fold and has eight helices and seven strands. A heptasaccharide moiety is also present, and amino acid residues that serve as the catalytic acid and base can be assigned to His32 and His91, respectively. Two "hypervariable" regions, known as HVa and HVb, are the proposed sites of S-allele discrimination during the self-incompatibility reaction, and in the S(F11)-RNase these are well separated from the active site. HVa and HVb are composed of a long, positively charged loop followed by a part of an alpha-helix and short, negatively charged alpha-helix, respectively. The S(F11)-RNase structure shows both regions are readily accessible to the solvent and hence could participate in the process of self/non-self discrimination between the S-RNase and an unknown pollen S-gene product(s) upon pollination.
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(as it appears on PubMed at http://www.pubmed.gov), where 11724536 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11724536}}
==About this Structure==
==About this Structure==
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[[Category: Yamashita, E.]]
[[Category: Yamashita, E.]]
[[Category: Self-incompatibility ribonuclease]]
[[Category: Self-incompatibility ribonuclease]]
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Revision as of 10:42, 1 July 2008

Image:1ioo.png

Template:STRUCTURE 1ioo

CRYSTAL STRUCTURE OF NICOTIANA ALATA GEMETOPHYTIC SELF-INCOMPATIBILITY ASSOCIATED SF11-RNASE

Template:ABSTRACT PUBMED 11724536

About this Structure

Full crystallographic information is available from OCA.

Reference

The 1.55 A resolution structure of Nicotiana alata S(F11)-RNase associated with gametophytic self-incompatibility., Ida K, Norioka S, Yamamoto M, Kumasaka T, Yamashita E, Newbigin E, Clarke AE, Sakiyama F, Sato M, J Mol Biol. 2001 Nov 16;314(1):103-12. PMID:11724536

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