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| - | [[Image:1ipp.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1ipp| PDB=1ipp | SCENE= }} | | {{STRUCTURE_1ipp| PDB=1ipp | SCENE= }} |
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| - | '''HOMING ENDONUCLEASE/DNA COMPLEX'''
| + | ===HOMING ENDONUCLEASE/DNA COMPLEX=== |
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| - | ==Overview==
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| - | Homing endonucleases are a diverse collection of proteins that are encoded by genes with mobile, self-splicing introns. They have also been identified in self-splicing inteins (protein introns). These enzymes promote the movement of the DNA sequences that encode them from one chromosome location to another; they do this by making a site-specific double-strand break at a target site in an allele that lacks the corresponding mobile intron. The target sites recognized by these small endonucleases are generally long (14-44 base pairs). Four families of homing endonucleases have been identified, including the LAGLIDADG, the His-Cys box, the GIY-YIG and the H-N-H endonucleases. The first identified His-Cys box homing endonuclease was I-PpoI from the slime mould Physarum polycephalum. Its gene resides in one of only a few nuclear introns known to exhibit genetic mobility. Here we report the structure of the I-PpoI homing endonuclease bound to homing-site DNA determined to 1.8 A resolution. I-PpoI displays an elongated fold of dimensions 25 x 35 x 80 A, with mixed alpha/beta topology. Each I-PpoI monomer contains three antiparallel beta-sheets flanked by two long alpha-helices and a long carboxy-terminal tail, and is stabilized by two bound zinc ions 15 A apart. The enzyme possesses a new zinc-bound fold and endonuclease active site. The structure has been determined in both uncleaved substrate and cleaved product complexes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9665136}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9665136 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9665136}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Transcription/dna]] | | [[Category: Transcription/dna]] |
| | [[Category: Zinc]] | | [[Category: Zinc]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 20:15:52 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 13:46:41 2008'' |
Revision as of 10:46, 1 July 2008
Template:STRUCTURE 1ipp
HOMING ENDONUCLEASE/DNA COMPLEX
Template:ABSTRACT PUBMED 9665136
About this Structure
1IPP is a Single protein structure of sequence from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
DNA binding and cleavage by the nuclear intron-encoded homing endonuclease I-PpoI., Flick KE, Jurica MS, Monnat RJ Jr, Stoddard BL, Nature. 1998 Jul 2;394(6688):96-101. PMID:9665136
Page seeded by OCA on Tue Jul 1 13:46:41 2008
